PMID- 8799210
OWN - NLM
STAT- MEDLINE
DCOM- 19961112
LR  - 20191101
IS  - 1355-0284 (Print)
IS  - 1355-0284 (Linking)
VI  - 2
IP  - 3
DP  - 1996 Jun
TI  - Clinical implications of nucleic acid amplification methods for the diagnosis of 
      viral infections of the nervous system.
PG  - 175-90
AB  - Amplification of viral nucleic acids from the cerebrospinal fluid (CSF) has 
      considerably improved the diagnosis of several acute, subacute and chronic viral 
      infections of the nervous system. In herpes simplex virus (HSV) encephalitis 
      (HSE) the polymerase chain reaction (PCR) has become the method of choice for the 
      rapid, non invasive diagnosis. Other herpes virus associated diseases which can 
      now be reliably diagnosed are encephalitis, ventriculoencephalitis, 
      polymyeloradiculitis, myelitis and an inflammatory polyradiculoneuropathy caused 
      by cytomegalovirus (CMV), HSV, varicella-zoster virus (VZV) or Epstein-Barr virus 
      (EBV), EBV associated primary B-cell-lymphoma of the brain, acute aseptic 
      meningitis in young adults allied with VZV, and meningoencephalitis with 
      recurrent seizures due to human herpes virus type 6 (HHV-6). In AIDS patients, 
      PCR has helped to differentiate lesions either due to the human immunodeficiency 
      virus (HIV) itself or to opportunistic infections such as progressive multifocal 
      leukoencephalopathy (PML) caused by JC virus (JCV) or CMV related complications. 
      HIV can be detected early in the course of infection in the CSF and the amount of 
      proviral DNA in CSF cells seems to be correlated with the severity and/or 
      progression of neurological signs and symptoms. Acute epidemic aseptic meningitis 
      caused by enterovirus infections can now be reliably diagnosed and typed by 
      reverse transcriptase PCR (RT-PCR). Meningitis cases caused by vaccination with 
      the Jeryl Lynn and Urabe vaccine strain of mumps virus have been identified using 
      RT-PCR and sequencing of the amplified products (amplicon).
FAU - Weber, T
AU  - Weber T
AD  - Department of Neurology, Georg-August-University, Gottingen, Germany.
FAU - Frye, S
AU  - Frye S
FAU - Bodemer, M
AU  - Bodemer M
FAU - Otto, M
AU  - Otto M
FAU - Luke, W
AU  - Luke W
LA  - eng
PT  - Journal Article
PT  - Review
PL  - United States
TA  - J Neurovirol
JT  - Journal of neurovirology
JID - 9508123
RN  - 0 (DNA, Viral)
RN  - 0 (Nucleic Acids)
SB  - IM
MH  - Central Nervous System/*virology
MH  - DNA, Viral/*isolation & purification
MH  - Encephalitis/*diagnosis/virology
MH  - Humans
MH  - Meningitis/*diagnosis/virology
MH  - Nucleic Acids/isolation & purification
MH  - Polymerase Chain Reaction
RF  - 170
EDAT- 1996/06/01 00:00
MHDA- 1996/06/01 00:01
CRDT- 1996/06/01 00:00
PHST- 1996/06/01 00:00 [pubmed]
PHST- 1996/06/01 00:01 [medline]
PHST- 1996/06/01 00:00 [entrez]
AID - 10.3109/13550289609146880 [doi]
PST - ppublish
SO  - J Neurovirol. 1996 Jun;2(3):175-90. doi: 10.3109/13550289609146880.