PMID- 8801709
OWN - NLM
STAT- MEDLINE
DCOM- 19961001
LR  - 20170214
IS  - 0300-9858 (Print)
IS  - 0300-9858 (Linking)
VI  - 33
IP  - 2
DP  - 1996 Mar
TI  - Comparison of the antigen distribution of two US porcine reproductive and 
      respiratory syndrome virus isolates with that of the Lelystad virus.
PG  - 159-70
AB  - One hundred 4-week-old cesarean-derived colostrum-deprived pigs were inoculated 
      with one of two different US porcine reproductive and respiratory syndrome virus 
      (PRRSV) isolates (VR2385, VR2431) or the European Lelystad virus to detect and 
      compare the location and amount of virus antigen. Interstitial pneumonia, 
      myocarditis, lymphadenopathy, and encephalitis were consistently seen in all 
      three groups; however, disease and lesions were more severe in the VR2385 group. 
      Immunohistochemical evaluation of formalin-fixed tissues revealed virus antigen 
      in alveolar macrophages in lungs of 22/25, 14/25, 14/25, and 0/25 of the VR2385, 
      VR2431, Lelystad, and control pigs, respectively. Follicular macrophages and 
      dendritic cells in the lymph nodes of 14/25, 10/25, 10/25, and 0/25 pigs from the 
      VR2385, VR2431, Lelystad, and control groups, respectively, stained positive for 
      virus antigen. Similar cells in the tonsils from 25/25, 21/25, 23/25, and 0/25 
      pigs from the VR2385, VR2431, Lelystad, and control groups, respectively, stained 
      positive for virus antigen. Other tissues and cells in which virus antigen was 
      detected included macrophages and endothelial cells in the heart, macrophages, 
      and interdigitating cells in the thymus, macrophages and dendritic cells in the 
      spleen and Peyer's patches, and macrophages in hepatic sinusoids, renal medullary 
      interstitium, and adrenal gland. PRRSV persisted in macrophages in the lung, 
      tonsil, lymph node, and spleen for at least 28 days. Significantly more PRRSV 
      antigen was detected in the lung (P < 0.01), lymph nodes (P < or = 0.05), and 
      tonsils (P < 0.05) of the VR2385 pigs than was detected in the same tissues of 
      the VR2431 and Lelystad pigs. The cell types in which PRRSV antigen was detected 
      and the distribution of PRRSV antigen-positive cells within particular tissues 
      and organs were generally similar for the different virus inoculation groups 
      despite differences in virulence of the isolates.
FAU - Halbur, P G
AU  - Halbur PG
AD  - Veterinary Diagnostic Laboratory, Iowa State University, Ames, USA.
FAU - Paul, P S
AU  - Paul PS
FAU - Frey, M L
AU  - Frey ML
FAU - Landgraf, J
AU  - Landgraf J
FAU - Eernisse, K
AU  - Eernisse K
FAU - Meng, X J
AU  - Meng XJ
FAU - Andrews, J J
AU  - Andrews JJ
FAU - Lum, M A
AU  - Lum MA
FAU - Rathje, J A
AU  - Rathje JA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Vet Pathol
JT  - Veterinary pathology
JID - 0312020
RN  - 0 (Antigens, Viral)
SB  - IM
MH  - Animals
MH  - Antigens, Viral/*analysis
MH  - Arterivirus/*immunology/isolation & purification/pathogenicity
MH  - Female
MH  - Infertility, Female/*veterinary/virology
MH  - Lung Diseases/*veterinary/virology
MH  - Swine
MH  - Swine Diseases/*virology
MH  - Syndrome
MH  - Tissue Distribution
EDAT- 1996/03/01 00:00
MHDA- 1996/03/01 00:01
CRDT- 1996/03/01 00:00
PHST- 1996/03/01 00:00 [pubmed]
PHST- 1996/03/01 00:01 [medline]
PHST- 1996/03/01 00:00 [entrez]
AID - 10.1177/030098589603300205 [doi]
PST - ppublish
SO  - Vet Pathol. 1996 Mar;33(2):159-70. doi: 10.1177/030098589603300205.