PMID- 8804796
OWN - NLM
STAT- MEDLINE
DCOM- 19970212
LR  - 20180216
IS  - 0009-3157 (Print)
IS  - 0009-3157 (Linking)
VI  - 42
IP  - 4
DP  - 1996 Jul-Aug
TI  - Fleroxacin enhancement of superoxide production by polymorphonuclear leukocytes: 
      the role of protein kinases.
PG  - 280-5
AB  - New quinolone (NQ) antimicrobials may influence the functions of 
      polymorphonuclear leukocyes (PMNs). Fleroxacin (FLRX), one of the newer NQs which 
      has a long half-life in blood and a strong bactericidal effect, was examined for 
      its influence on superoxide production by PMNs. Augmentation of superoxide 
      production by PMNs when stimulated with phorbol myristate acetate (PMA) and 
      formyl-methionyl-leucyl-phenylalanine (fMLP) was observed following the addition 
      of 25, 50, 100 and 200 micrograms/ml of FLRX. In addition, the effects of 
      staurosporine and H-7, inhibitors of protein kinase C (PKC), and of genistein, a 
      tyrosine kinase (TK) inhibitor, on FLRX-enhanced superoxide production were 
      examined. Superoxide production augmented by FLRX was diminished by the addition 
      of staurosporine and H-7, when PMNs were stimulated with PMA, and by the addition 
      of genistein, when PMNs were stimulated with fMLP. These results suggest that 
      FLRX augments superoxide production by PMNs through enhancing the activities of 
      phosphorylation by PKC or TK within the signal transduction pathway in PMNs.
FAU - Matsumoto, T
AU  - Matsumoto T
AD  - Department of Urology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
FAU - Takahashi, K
AU  - Takahashi K
FAU - Nagafuji, T
AU  - Nagafuji T
FAU - Kubo, S
AU  - Kubo S
FAU - Sakumoto, M
AU  - Sakumoto M
FAU - Mochida, O
AU  - Mochida O
FAU - Sakamoto, Y
AU  - Sakamoto Y
FAU - Mizunoe, Y
AU  - Mizunoe Y
FAU - Kumazawa, J
AU  - Kumazawa J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Chemotherapy
JT  - Chemotherapy
JID - 0144731
RN  - 0 (Anti-Infective Agents)
RN  - 0 (Carcinogens)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Isoflavones)
RN  - 11062-77-4 (Superoxides)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 84477-87-2 (1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine)
RN  - DH2M523P0H (Genistein)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - H88EPA0A3N (Staurosporine)
RN  - N804LDH51K (Fleroxacin)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine/pharmacology
MH  - Anti-Infective Agents/*pharmacology
MH  - Carcinogens/pharmacology
MH  - Enzyme Inhibitors/pharmacology
MH  - Fleroxacin/*pharmacology
MH  - Genistein
MH  - Humans
MH  - In Vitro Techniques
MH  - Isoflavones/pharmacology
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/drug effects/enzymology/*metabolism
MH  - Protein Kinase C/antagonists & inhibitors/blood/physiology
MH  - Protein Kinases/blood/*physiology
MH  - Protein-Tyrosine Kinases/antagonists & inhibitors/blood/physiology
MH  - Signal Transduction/drug effects
MH  - Staurosporine/pharmacology
MH  - Superoxides/*blood
MH  - Tetradecanoylphorbol Acetate/pharmacology
EDAT- 1996/07/01 00:00
MHDA- 1996/07/01 00:01
CRDT- 1996/07/01 00:00
PHST- 1996/07/01 00:00 [pubmed]
PHST- 1996/07/01 00:01 [medline]
PHST- 1996/07/01 00:00 [entrez]
AID - 10.1159/000239456 [doi]
PST - ppublish
SO  - Chemotherapy. 1996 Jul-Aug;42(4):280-5. doi: 10.1159/000239456.