PMID- 8806181
OWN - NLM
STAT- MEDLINE
DCOM- 19961204
LR  - 20190909
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 42
IP  - 1-2
DP  - 1996 Jun
TI  - Porcine reproductive and respiratory syndrome virus enters cells through a low 
      pH-dependent endocytic pathway.
PG  - 137-47
AB  - The entry pathway of porcine reproductive and respiratory syndrome virus (PRRSV) 
      into MARC-145 cells was investigated using a variety of drugs that interfere with 
      the pH of intracellular vesicles by different mechanisms. Virus entry was 
      assessed by measuring viral RNA replication or production of infectious virus. 
      Chloroquine, ammonium chloride and bafilomycin A1 inhibited RNA replication or 
      production of infectious virus in a dose-dependent manner. These drugs inhibited 
      virus replication when added to the cells prior to, at infection or soon after 
      infection. Moreover, the effect of chloroquine on PRRSV replication was 
      reversible under acidic conditions of the media. Taken together, these results 
      indicated that a low pH was required during virus entry. Electron microscopic 
      data showed virus particles at the cell surface or within small vesicles which 
      were circumscribed by a clathrin-like zone. In addition, the number of 
      PRRSV-infected cells was decreased in the presence of cytochalasin B and 
      phenylarsine oxide. Thus, we concluded that PRRSV entry might occur through a 
      microfilament-dependent endocytic mechanism in which a low pH is necessary for 
      proper virus uncoating.
FAU - Kreutz, L C
AU  - Kreutz LC
AD  - Virology Swine Research Unit, National Animal Disease Center, USDA, Agricultural 
      Research Service, Ames, Iowa 50010, USA.
FAU - Ackermann, M R
AU  - Ackermann MR
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Macrolides)
RN  - 01Q9PC255D (Ammonium Chloride)
RN  - 63231-63-0 (RNA)
RN  - 886U3H6UFF (Chloroquine)
RN  - 88899-55-2 (bafilomycin A1)
SB  - IM
MH  - Actin Cytoskeleton/metabolism
MH  - Ammonium Chloride/pharmacology
MH  - Animals
MH  - Anti-Bacterial Agents/pharmacology
MH  - Cell Line
MH  - Chloroquine/pharmacology
MH  - Endocytosis
MH  - Hydrogen-Ion Concentration
MH  - *Macrolides
MH  - Porcine respiratory and reproductive syndrome virus/drug effects/isolation & 
      purification/*metabolism/physiology
MH  - RNA/biosynthesis
MH  - Swine/virology
MH  - Virus Replication
EDAT- 1996/06/01 00:00
MHDA- 1996/06/01 00:01
CRDT- 1996/06/01 00:00
PHST- 1996/06/01 00:00 [pubmed]
PHST- 1996/06/01 00:01 [medline]
PHST- 1996/06/01 00:00 [entrez]
AID - 0168-1702(96)01313-5 [pii]
AID - 10.1016/0168-1702(96)01313-5 [doi]
PST - ppublish
SO  - Virus Res. 1996 Jun;42(1-2):137-47. doi: 10.1016/0168-1702(96)01313-5.