PMID- 8806183
OWN - NLM
STAT- MEDLINE
DCOM- 19961204
LR  - 20190909
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 42
IP  - 1-2
DP  - 1996 Jun
TI  - Phylogenetic relationships of european strains of porcine reproductive and 
      respiratory syndrome virus (PRRSV) inferred from DNA sequences of putative ORF-5 
      and ORF-7 genes.
PG  - 159-65
AB  - The complete ORF-5 gene and a fragment of the ORF-7 gene from 14 different 
      European porcine reproductive and respiratory syndrome virus (PRRSV) isolates 
      were amplified by reverse transcriptase polymerase chain reaction (RT-PCR), and 
      their nucleotide sequences were determined. The ORF-7 gene displayed nucleotide 
      and amino acid identities of 94.1-99.6% and 95.3-100% among isolates from 
      different countries. The ORF-5 gene showed higher nucleotide (87.1-99.2% 
      identity) and amino acid (-88% identity) variability. The resulting sequences 
      were aligned with other European and North American PRRSV strains and 
      phylogenetic relationships among these strains were established by the maximum 
      parsimony method. The phylogenetic trees inferred from both genes were in 
      agreement and showed that European and North American PRRSV strains clearly 
      represent two different genotypes. According to both trees, there is a perfect 
      correlation between strains and the countries in which they were isolated. 
      Additionally, the phylogenetic position of European and North American PRRSV 
      strains within the recently proposed family Arteriviridae was also analyzed.
FAU - Suarez, P
AU  - Suarez P
AD  - Departamento de Patologia Animal I (Sanidad Animal), Facultad de Veterinaria, 
      Universidad Complutense, Madrid, Spain.
FAU - Zardoya, R
AU  - Zardoya R
FAU - Martin, M J
AU  - Martin MJ
FAU - Prieto, C
AU  - Prieto C
FAU - Dopazo, J
AU  - Dopazo J
FAU - Solana, A
AU  - Solana A
FAU - Castro, J M
AU  - Castro JM
LA  - eng
SI  - GENBANK/U40687
SI  - GENBANK/U40688
SI  - GENBANK/U40689
SI  - GENBANK/U40690
SI  - GENBANK/U40691
SI  - GENBANK/U40692
SI  - GENBANK/U40694
SI  - GENBANK/U40695
SI  - GENBANK/U40696
SI  - GENBANK/U40697
SI  - GENBANK/U40698
SI  - GENBANK/U40699
SI  - GENBANK/U40700
SI  - GENBANK/U40701
SI  - GENBANK/U40721
SI  - GENBANK/U40722
SI  - GENBANK/U40723
SI  - GENBANK/U40724
SI  - GENBANK/U40725
SI  - GENBANK/U40726
SI  - GENBANK/U40727
SI  - GENBANK/U40778
SI  - GENBANK/U40779
SI  - GENBANK/U40780
SI  - GENBANK/U40781
SI  - GENBANK/U40782
SI  - GENBANK/U40783
SI  - GENBANK/U40784
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - *DNA, Viral
MH  - Molecular Sequence Data
MH  - *Open Reading Frames
MH  - Phylogeny
MH  - Polymerase Chain Reaction
MH  - Porcine respiratory and reproductive syndrome virus/*classification/*genetics
MH  - Swine/virology
EDAT- 1996/06/01 00:00
MHDA- 1996/06/01 00:01
CRDT- 1996/06/01 00:00
PHST- 1996/06/01 00:00 [pubmed]
PHST- 1996/06/01 00:01 [medline]
PHST- 1996/06/01 00:00 [entrez]
AID - 0168-1702(95)01305-9 [pii]
AID - 10.1016/0168-1702(95)01305-9 [doi]
PST - ppublish
SO  - Virus Res. 1996 Jun;42(1-2):159-65. doi: 10.1016/0168-1702(95)01305-9.