PMID- 8806771
OWN - NLM
STAT- MEDLINE
DCOM- 19961029
LR  - 20190629
IS  - 0003-9861 (Print)
IS  - 0003-9861 (Linking)
VI  - 333
IP  - 1
DP  - 1996 Sep 1
TI  - Transforming growth factor beta 1 increases the synthesis and shedding of the 
      melanoma-specific proteoglycan in human melanoma cells.
PG  - 198-206
AB  - Melanoma cells produce a cell-specific proteoglycan, mel-PG, which is an integral 
      chondroitin sulfate proteoglycan that can be released into the medium as a result 
      of the proteolytic cleavage of the trans-membrane form. The effect of 
      transforming growth factor beta 1 (TGF-beta 1) on proteoglycan production was 
      studied in three melanoma cell lines at various stages of differentiation: 
      SK-mel-1.36-1-5 (early), SK-mel-3.44 (intermediate), and SK-mel-23 (late). The 
      main effect of TGF-beta 1 was to increase the synthesis and shedding of mel-PG 
      into the medium without affecting the amount present in the cell membranes nor 
      the balance between the proteoglycan and the glycoprotein forms of mel-PG. After 
      TGF-beta 1 treatment, there was an increase in the amount of mel-PG present in 
      the medium as observed in metabolic labeling, immunoprecipitation, and 
      pulse-chase experiments. This effect was more pronounced in the SK-mel-1.36-1-5 
      than in the SK-mel-3.44 cell line, whereas the SK-mel-23 cells did not contain 
      mel-PG either in the presence or in the absence of TGF-beta 1. Characterization 
      of mel-PG purified from the medium from control and TGF-beta 1-treated cells 
      showed that the factor increased slightly the GAG chain length in SK-mel-1.36-1-5 
      but not in SK-mel-3.44 cells, without modifying the degree of sulfation.
FAU - Heredia, A
AU  - Heredia A
AD  - Departament de Bioquimica i Biologia Molecular, Facultat de Veterinaria, 
      Universitat Autonoma de Barcelona, Bellaterra, Spain.
FAU - Villena, J
AU  - Villena J
FAU - Romaris, M
AU  - Romaris M
FAU - Molist, A
AU  - Molist A
FAU - Bassols, A
AU  - Bassols A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arch Biochem Biophys
JT  - Archives of biochemistry and biophysics
JID - 0372430
RN  - 0 (Proteoglycans)
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Cell Differentiation
MH  - Cell Membrane/drug effects/metabolism
MH  - Extracellular Space/drug effects/metabolism
MH  - Humans
MH  - Melanoma/*metabolism/pathology
MH  - Molecular Structure
MH  - Proteoglycans/*biosynthesis/chemistry/metabolism
MH  - Transforming Growth Factor beta/*pharmacology
MH  - Tumor Cells, Cultured
EDAT- 1996/09/01 00:00
MHDA- 1996/09/01 00:01
CRDT- 1996/09/01 00:00
PHST- 1996/09/01 00:00 [pubmed]
PHST- 1996/09/01 00:01 [medline]
PHST- 1996/09/01 00:00 [entrez]
AID - S0003986196903811 [pii]
AID - 10.1006/abbi.1996.0381 [doi]
PST - ppublish
SO  - Arch Biochem Biophys. 1996 Sep 1;333(1):198-206. doi: 10.1006/abbi.1996.0381.