PMID- 8807772
OWN - NLM
STAT- MEDLINE
DCOM- 19970121
LR  - 20190830
IS  - 0165-2427 (Print)
IS  - 0165-2427 (Linking)
VI  - 52
IP  - 1-2
DP  - 1996 Jun 15
TI  - Flow cytometric detection of activation-induced cell death (apoptosis) in 
      peripheral blood lymphocyte subpopulations from healthy cats.
PG  - 1-14
AB  - Human peripheral blood lymphocytes (PBLs) from healthy individuals are resistant 
      to in vitro-induced apoptosis, but activated human lymphocytes can readily 
      undergo apoptosis. The activation of human lymphocytes is accompanied by the 
      upregulation of a cell surface antigen, the major histocompatibility complex 
      (MHC) class II-antigen. Only a minority of PBLs are usually MHC class 
      II-antigen-positive in healthy humans. In contrast, in healthy cats the majority 
      of feline PBLs are MHC class II-antigen-positive. We have, therefore, 
      investigated the sensitivity of feline peripheral blood lymphocytes obtained from 
      specified pathogen free (SPF) cats to the induction of apoptosis. Feline PBLs 
      from SPF cats (n = 16) and human PBLs from healthy donors (n = 2) were isolated. 
      After short-term culture, cells were examined for the presence of fragmented DNA 
      as a result of apoptosis by a DNA agarose gel electrophoresis method and for the 
      presence of DNA double strand breaks by in situ 3' end labeling. In addition, 
      relative DNA content per cell was flow cytometrically determined using propidium 
      iodide (PI) or 7-actinomycin-D (7-AAD) and apoptotic cells were identified on the 
      basis of a reduced DNA content. Cell surface antigens and cellular DNA were 
      analyzed simultaneously by dual-color flow cytometric analyses in order to study 
      lymphocyte subsets. Single- and dual-color analysis revealed that, in contrast to 
      human lymphocytes, feline lymphocytes rapidly underwent apoptosis when cultured 
      overnight in medium. Furthermore, the majority of apoptotic cells was found 
      within the MHC II-positive cell subject.
FAU - Holznagel, E
AU  - Holznagel E
AD  - Department of Internal Veterinary Medicine, University of Zurich, Switzerland.
FAU - Hofmann-Lehmann, R
AU  - Hofmann-Lehmann R
FAU - Allenspach, K
AU  - Allenspach K
FAU - Huttner, S
AU  - Huttner S
FAU - Willett, B
AU  - Willett B
FAU - Groscurth, P
AU  - Groscurth P
FAU - Niederer, E
AU  - Niederer E
FAU - Lutz, H
AU  - Lutz H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Histocompatibility Antigens Class II)
SB  - IM
MH  - Animals
MH  - Apoptosis/*immunology
MH  - Cats
MH  - Flow Cytometry
MH  - Histocompatibility Antigens Class II/immunology
MH  - Humans
MH  - Lymphocyte Activation/*immunology
MH  - Lymphocyte Subsets/*immunology
MH  - Reproducibility of Results
EDAT- 1996/06/15 00:00
MHDA- 1996/06/15 00:01
CRDT- 1996/06/15 00:00
PHST- 1996/06/15 00:00 [pubmed]
PHST- 1996/06/15 00:01 [medline]
PHST- 1996/06/15 00:00 [entrez]
AID - 0165-2427(95)05541-X [pii]
AID - 10.1016/0165-2427(95)05541-x [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 1996 Jun 15;52(1-2):1-14. doi: 
      10.1016/0165-2427(95)05541-x.