PMID- 8811027
OWN - NLM
STAT- MEDLINE
DCOM- 19961105
LR  - 20071114
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 77 ( Pt 9)
DP  - 1996 Sep
TI  - Quantification of human herpesvirus 6 in immunocompetent persons and post-mortem 
      tissues from AIDS patients by PCR.
PG  - 2271-5
AB  - A quantitative competitive PCR assay for human herpesvirus 6 (HHV-6) was 
      developed. Firstly, viral burden was determined in the blood of 25 healthy 
      persons. Using 1 microgram of DNA, the prevalence of HHV-6 was 36% (9/25). Eight 
      persons had viral loads of < or = 32 HHV-6 genomes/microgram DNA. The viral 
      burden in the ninth individual was 1.2 x 10(6) HHV-6 genome copies/microgram DNA, 
      which remained constant over a period of 10 months. This demonstrates the 
      persistence of a high HHV-6 load in the absence of apparent disease. Secondly, 
      HHV-6 burden was determined in 100 post-mortem tissues from seven AIDS patients 
      and three controls. For all tissues combined, there was a statistically 
      significant higher median viral load in AIDS patients (56 copies/microgram DNA, 
      range 0-43321) compared to controls (10 copies/microgram DNA, range 0-423) (P = 
      0.04). The precision and reproducibility of this assay will allow hypotheses 
      concerning the pathogenic potential of HHV-6 to be tested quantitatively.
FAU - Clark, D A
AU  - Clark DA
AD  - Department of Virology, Royal Free Hospital School of Medicine, London, UK.
FAU - Ait-Khaled, M
AU  - Ait-Khaled M
FAU - Wheeler, A C
AU  - Wheeler AC
FAU - Kidd, I M
AU  - Kidd IM
FAU - McLaughlin, J E
AU  - McLaughlin JE
FAU - Johnson, M A
AU  - Johnson MA
FAU - Griffiths, P D
AU  - Griffiths PD
FAU - Emery, V C
AU  - Emery VC
LA  - eng
GR  - AI33389/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (DNA, Viral)
SB  - IM
MH  - AIDS-Related Opportunistic Infections/immunology/*virology
MH  - DNA, Viral/analysis
MH  - Herpesviridae Infections/*complications/immunology/virology
MH  - Herpesvirus 6, Human/genetics/*isolation & purification
MH  - Humans
MH  - Immunocompetence
MH  - Polymerase Chain Reaction
MH  - Viral Load
EDAT- 1996/09/01 00:00
MHDA- 1996/09/01 00:01
CRDT- 1996/09/01 00:00
PHST- 1996/09/01 00:00 [pubmed]
PHST- 1996/09/01 00:01 [medline]
PHST- 1996/09/01 00:00 [entrez]
AID - 10.1099/0022-1317-77-9-2271 [doi]
PST - ppublish
SO  - J Gen Virol. 1996 Sep;77 ( Pt 9):2271-5. doi: 10.1099/0022-1317-77-9-2271.