PMID- 8813710
OWN - NLM
STAT- MEDLINE
DCOM- 19961210
LR  - 20190818
IS  - 0300-8177 (Print)
IS  - 0300-8177 (Linking)
VI  - 159
IP  - 1
DP  - 1996 Jun 7
TI  - Enzymes of carbohydrate metabolism in young and adult rats fed diets differing in 
      fat and carbohydrate.
PG  - 55-63
AB  - Glycogen content as well as glycolytic, gluconeogenic and fatty acid synthesis 
      enzyme activities were monitored in young and adult male rats fed diets differing 
      in fat content: 11% (low), 22% (medium) and 42% (high) of total energy from fat. 
      The results showed significant differences in the responses of young and adult 
      rats to changes in dietary fat and carbohydrate. In young animals, increasing 
      dietary fat decreased total liver glycogen phosphorylase (GP), pyruvate kinase 
      (PK), glycerol 3-phosphate dehydrogenase, glucose 6-phosphate dehydrogenase, 
      malic enzyme (ME), ATP-citrate lyase (ATP-CL) and fatty acid synthase (FAS). 
      Increasing dietary fat also affected enzyme levels in other tissues: hexokinase 
      (HK) and pyruvate dehydrogenase (PDH) activities decreased whereas skeletal 
      muscle PK activity increased. The pattern of enzyme changes was similar in livers 
      of fed adults with the exception that liver GP was not affected by dietary 
      manipulations. Overnight food deprivation decreased liver glucokinase (GK), ME, 
      ATP-CL, and FAS activities and increased liver phosphoenolpyruvate carboxykinase 
      (PEPCK) and phosphofructokinase in both young and adult animals. In young 
      animals, food deprivation also: (i) reduced liver GK and PK, (ii) increased 
      kidney PEPCK, (iii) decreased muscle PEPCK and (iv) decreased kidney PDH. 
      Food-deprived adults had increased skeletal muscle PEPCK and kidney glycogen 
      synthetase as well as decreased kidney PEPCK muscle GP activity. These 
      differences suggest that young animals are somewhat more responsive to changes in 
      dietary manipulations. They also show that overnight food restriction causes a 
      more profound metabolic re-organization in younger than in older animals.
FAU - Brooks, S P
AU  - Brooks SP
AD  - Nutrition Research Division, Health protection Branch, Health Canada. Ottawa, 
      Ontario, Canada.
FAU - Lampi, B J
AU  - Lampi BJ
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Dietary Fats)
RN  - 0 (Enzymes)
RN  - 0 (Fatty Acids)
RN  - 9005-79-2 (Glycogen)
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - Dietary Carbohydrates/*pharmacology
MH  - Dietary Fats/*pharmacology
MH  - Enzymes/*biosynthesis
MH  - Fatty Acids/*biosynthesis
MH  - Food Deprivation/physiology
MH  - *Gluconeogenesis
MH  - Glycogen/*metabolism
MH  - Kidney/enzymology
MH  - Liver/enzymology
MH  - Male
MH  - Muscle, Skeletal/enzymology
MH  - Plasma/enzymology
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 1996/06/07 00:00
MHDA- 1996/06/07 00:01
CRDT- 1996/06/07 00:00
PHST- 1996/06/07 00:00 [pubmed]
PHST- 1996/06/07 00:01 [medline]
PHST- 1996/06/07 00:00 [entrez]
AID - 10.1007/BF00226063 [doi]
PST - ppublish
SO  - Mol Cell Biochem. 1996 Jun 7;159(1):55-63. doi: 10.1007/BF00226063.