PMID- 8814331
OWN - NLM
STAT- MEDLINE
DCOM- 19961104
LR  - 20190723
IS  - 0022-1759 (Print)
IS  - 0022-1759 (Linking)
VI  - 195
IP  - 1-2
DP  - 1996 Sep 9
TI  - Alternative assay procedures for cytokines and soluble receptors of the IL-6 
      family.
PG  - 153-9
AB  - Human hepatoma cells (HepG2 cells) were transfected with expression vectors for 
      human IL-6 (hIL-6) and rat IL-6R (rIL-6-R). The cell lines were used for testing 
      the biological activity of different IL-6 species, soluble hIL-6R (shIL-6R) and 
      some members of the IL-6 cytokine family by means of an ELISA procedure. The 
      assay is based on induction of the gene expression of the acute phase protein 
      haptoglobin in hepatoma cells and provides an alternative bioassay taking 
      advantage of the hepatocyte stimulatory activity of IL-6 (as opposed to the B9 
      proliferative assay). A dose-response experiment with IL-6 showed that 
      half-maximal stimulation was achieved with approx. 5 ng/ml of hIL-6 in HepG2 
      cells and with 5-10 ng/ml muIL-6 in HepG2-rIL-6R cells after 24 h. The same 
      response was achieved with 10 ng/ml shIL-6R in HepG2-IL6 cells. In conclusion, 
      the assay is fast and reliable and might be adopted for other cytokines and 
      receptors with hepatocyte stimulating activity.
FAU - Oppmann, B
AU  - Oppmann B
AD  - I. Department of Medicine, Johannes Gutenberg University of Mainz, Germany.
FAU - Stoyan, T
AU  - Stoyan T
FAU - Fischer, M
AU  - Fischer M
FAU - Voltz, N
AU  - Voltz N
FAU - Marz, P
AU  - Marz P
FAU - Rose-John, S
AU  - Rose-John S
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Immunol Methods
JT  - Journal of immunological methods
JID - 1305440
RN  - 0 (Antigens, CD)
RN  - 0 (Interleukin-6)
RN  - 0 (Receptors, Interleukin)
RN  - 0 (Receptors, Interleukin-6)
SB  - IM
MH  - Animals
MH  - Antigens, CD/*analysis/genetics
MH  - Biological Assay
MH  - Gene Transfer Techniques
MH  - Humans
MH  - Interleukin-6/*analysis/genetics
MH  - Rats
MH  - Receptors, Interleukin/*analysis/genetics
MH  - Receptors, Interleukin-6
MH  - Tumor Cells, Cultured
EDAT- 1996/09/09 00:00
MHDA- 1996/09/09 00:01
CRDT- 1996/09/09 00:00
PHST- 1996/09/09 00:00 [pubmed]
PHST- 1996/09/09 00:01 [medline]
PHST- 1996/09/09 00:00 [entrez]
AID - 0022-1759(96)00110-X [pii]
AID - 10.1016/0022-1759(96)00110-x [doi]
PST - ppublish
SO  - J Immunol Methods. 1996 Sep 9;195(1-2):153-9. doi: 10.1016/0022-1759(96)00110-x.