PMID- 8816059
OWN - NLM
STAT- MEDLINE
DCOM- 19961211
LR  - 20061115
IS  - 0047-1860 (Print)
IS  - 0047-1860 (Linking)
VI  - 44
IP  - 8
DP  - 1996 Aug
TI  - [Progress in diagnosis of leukemia].
PG  - 736-42
AB  - Recent advances in molecular biological techniques have contributed to the 
      tremendous progression made in the field of diagnosis of leukemia. Discovery of 
      T- or B-lymphocyte associated genes, tumor specific genes and genes involved in 
      chromosomal translocation has made it possible to detect leukemia cells by 
      Southern blotting, PCR, RT-PCR or fluorescence in situ hybridization (FISH). The 
      recently developed FISH is a simple, rapid and accurate method and requires a 
      very small amount of specimen (about 500-1000 cells). It is possible to obtain 
      results within 48 hours of sampling. This lecture were focused on two topics; 1) 
      The application of FISH method in the diagnosis of leukemia using three types of 
      probes (whole chromosome painting probe, centromeric probes and oncogene specific 
      probes) and their combinations. 2) Clarification of concepts made by molecular 
      biology especially in Philadelphia chromosome positive leukemia, Ph-negative 
      chronic myelocytic leukemia, endemic/sporadic type of Burkitt's lymphoma, 
      biphenotypic leukemia and leukemia with specific translocations.
FAU - Kamada, N
AU  - Kamada N
AD  - Department of Cancer Cytogenetics, Hiroshima University.
FAU - Ishimae, M
AU  - Ishimae M
FAU - Eguchi, M
AU  - Eguchi M
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PT  - Review
PL  - Japan
TA  - Rinsho Byori
JT  - Rinsho byori. The Japanese journal of clinical pathology
JID - 2984781R
RN  - 0 (DNA Probes)
SB  - IM
MH  - DNA Probes
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia/*diagnosis/genetics
MH  - Oncogenes
MH  - Translocation, Genetic
RF  - 8
EDAT- 1996/08/01 00:00
MHDA- 1996/08/01 00:01
CRDT- 1996/08/01 00:00
PHST- 1996/08/01 00:00 [pubmed]
PHST- 1996/08/01 00:01 [medline]
PHST- 1996/08/01 00:00 [entrez]
PST - ppublish
SO  - Rinsho Byori. 1996 Aug;44(8):736-42.