PMID- 8816240
OWN - NLM
STAT- MEDLINE
DCOM- 19970219
LR  - 20131121
IS  - 0736-6205 (Print)
IS  - 0736-6205 (Linking)
VI  - 21
IP  - 1
DP  - 1996 Jul
TI  - Application of 5-bromo-2'deoxyuridine as a label for in situ hybridization in 
      chromosome microdissection and painting, and 3' OH DNA end labeling for 
      apoptosis.
PG  - 82-6
AB  - We have utilized 5-bromo-2'deoxyuridine (BrdU) substituted DNA as a probe for a 
      number of applications including, principally, for chromosome painting by 
      fluorescence in situ hybridization (FISH) but also for DNA end-labeling to detect 
      apoptotic cell death and for filter hybridization. Br-dUTP was used as a 
      substitute for biotin or digoxigenin-dUTP in probe labeling techniques, such as 
      random priming, nick translation, end-labeling or PCR. An especially useful 
      application is that it may be incorporated into probe DNA while cells or plasmids 
      in bacteria are growing in the presence of BrdU. This can be particularly 
      advantageous when large quantities of probe are needed, since the cost per mole 
      of digoxigenin-dUTP or biotin-dUTP is nearly 1000 times that of Br-dUTP. Also, if 
      probe is prepared by growth in BrdU, the difference in cost to prepare equal 
      quantities of labeled DNA is more than 10,000 times greater for biotin-dUTP.
FAU - Muhlmann-Diaz, M C
AU  - Muhlmann-Diaz MC
AD  - Colorado State University, Fort Collins 80523-1673, USA.
FAU - Dullea, R G
AU  - Dullea RG
FAU - Bedford, J S
AU  - Bedford JS
LA  - eng
GR  - CA09236/CA/NCI NIH HHS/United States
GR  - CA18023/CA/NCI NIH HHS/United States
GR  - CA49501/CA/NCI NIH HHS/United States
PT  - Research Support, U.S. Gov't, P.H.S.
PT  - Technical Report
PL  - England
TA  - Biotechniques
JT  - BioTechniques
JID - 8306785
RN  - 0 (DNA Probes)
RN  - 9007-49-2 (DNA)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - *Bromodeoxyuridine/analysis
MH  - CHO Cells
MH  - Chromosomes/chemistry/*ultrastructure
MH  - Chromosomes, Human, Pair 11/chemistry
MH  - Cricetinae
MH  - DNA/*analysis
MH  - DNA Probes
MH  - HeLa Cells
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lymphocytes/radiation effects/ultrastructure
MH  - Microsurgery
MH  - Polymerase Chain Reaction
OTO - NASA
OT  - Non-programmatic
EDAT- 1996/07/01 00:00
MHDA- 1996/07/01 00:01
CRDT- 1996/07/01 00:00
PHST- 1996/07/01 00:00 [pubmed]
PHST- 1996/07/01 00:01 [medline]
PHST- 1996/07/01 00:00 [entrez]
PST - ppublish
SO  - Biotechniques. 1996 Jul;21(1):82-6.