PMID- 8816630
OWN - NLM
STAT- MEDLINE
DCOM- 19961024
LR  - 20220331
IS  - 0015-0282 (Print)
IS  - 0015-0282 (Linking)
VI  - 66
IP  - 4
DP  - 1996 Oct
TI  - The relationship between acridine orange fluorescence of sperm nuclei and the 
      fertilizing ability of human sperm.
PG  - 634-9
AB  - OBJECTIVE: To determine whether the outcome of IVF can be predicted by acridine 
      orange (AO) nuclear fluorescence of sperm. DESIGN: Based on the fact that AO 
      nuclear fluorescence color after acid treatment reflects maturity (green 
      fluorescence) or immaturity (yellow to red fluorescence) of spermatozoa, the 
      relationships between sperm maturity and the outcome of IVF, subzonal 
      insemination, or intracytoplasmic sperm injection (ICSI) were investigated. 
      SETTING: The IVF program at the Obstetrics and Gynecology Hospital, Fukushima 
      Medical College. PATIENTS: Sixty-eight patients undergoing 68 IVF treatment 
      cycles. MAIN OUTCOME MEASURES: Acridine orange fluorescence of sperm nuclei and 
      successful oocyte fertilization. RESULTS: conventional semen parameters (sperm 
      concentration and percentages of motile or morphologically normal spermatozoa in 
      semen) did not correlate with the incidence of spermatozoa with green AO 
      fluorescent (mature) nuclei. When > or = 50% of spermatozoa in semen samples 
      exhibited green AO nuclear fluorescence, IVF was always successful. When green AO 
      nuclear fluorescence was < 50%, only 39% of IVF treatment cycles (13/33) were 
      successful. Only green AO fluorescent spermatozoa were able to bind efficiently 
      human zona pellucida. When the incidence of green AO fluorescent spermatozoa was 
      < 50%, no pregnancy resulted even though an average of 26% of the oocytes could 
      be fertilized by ICSI. CONCLUSIONS: The spermatozoa which fertilized oocytes in 
      vivo and in IVF were limited to those whose nuclei exhibited green AO 
      fluorescence. Intracytoplasmic sperm injection may be the method of choice when 
      the incidence of green AO nuclear fluorescence is low regardless of the results 
      of semen analysis.
FAU - Hoshi, K
AU  - Hoshi K
AD  - Department of Obstetrics and Gynecology, Fukushima Medical College, Japan.
FAU - Katayose, H
AU  - Katayose H
FAU - Yanagida, K
AU  - Yanagida K
FAU - Kimura, Y
AU  - Kimura Y
FAU - Sato, A
AU  - Sato A
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - *Acridine Orange
MH  - Female
MH  - *Fertilization in Vitro
MH  - Fluorescence
MH  - Humans
MH  - Male
MH  - Pregnancy
MH  - Spermatozoa/*physiology
EDAT- 1996/10/01 00:00
MHDA- 1996/10/01 00:01
CRDT- 1996/10/01 00:00
PHST- 1996/10/01 00:00 [pubmed]
PHST- 1996/10/01 00:01 [medline]
PHST- 1996/10/01 00:00 [entrez]
AID - S0015-0282(16)58581-1 [pii]
AID - 10.1016/s0015-0282(16)58581-1 [doi]
PST - ppublish
SO  - Fertil Steril. 1996 Oct;66(4):634-9. doi: 10.1016/s0015-0282(16)58581-1.