PMID- 8817071 OWN - NLM STAT- MEDLINE DCOM- 19961127 LR - 20191101 IS - 0967-3849 (Print) IS - 0967-3849 (Linking) VI - 4 IP - 4 DP - 1996 Jun TI - Fluorescence in situ hybridization with rDNA probes on chromosomes of two nucleolus organizer region phenotypes of a species of Eigenmannia (Pisces, Gymnotoidei, Sternopygidae). PG - 301-5 AB - Nucleolus organizer regions (NORs) were analysed in two related and geographically close populations of Eigenmannia sp.1 (Pisces, Gymnotoidei, Sternopygidae) using silver staining and fluorescence in situ hybridization (FISH). The two populations differed in their Ag-NOR phenotypes, displaying fixed differences in the NOR-bearing chromosome pairs. FISH with rDNA probes showed that these differences were due to the location of rDNA cistrons. This finding, showing fixed NOR differences between two populations belonging to the same species in a connected river system, is highly significant in terms of evolutionary change, possibly indicating an initial step of genetic differentiation. This result also has important implications from the cytosystematic point of view, as NORs usually have a very constant karyotypic location in fish species and have been used as species-specific chromosome markers. FAU - de Almeida-Toledo, L F AU - de Almeida-Toledo LF AD - Departamento de Biologia, Universidade de Sao Paulo, Brazil. FAU - Stocker, A J AU - Stocker AJ FAU - Foresti, F AU - Foresti F FAU - Toledo-Filho, S de A AU - Toledo-Filho Sde A LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Netherlands TA - Chromosome Res JT - Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology JID - 9313452 RN - 0 (DNA Probes) RN - 0 (DNA, Ribosomal) SB - IM MH - Animals MH - Brazil MH - Chromosome Banding MH - *DNA Probes MH - *DNA, Ribosomal MH - Electric Fish/*genetics MH - Female MH - In Situ Hybridization, Fluorescence/*methods MH - Karyotyping MH - Male MH - Nucleolus Organizer Region/*genetics MH - Phenotype EDAT- 1996/06/01 00:00 MHDA- 1996/06/01 00:01 CRDT- 1996/06/01 00:00 PHST- 1996/06/01 00:00 [pubmed] PHST- 1996/06/01 00:01 [medline] PHST- 1996/06/01 00:00 [entrez] AID - 10.1007/BF02263681 [doi] PST - ppublish SO - Chromosome Res. 1996 Jun;4(4):301-5. doi: 10.1007/BF02263681.