PMID- 8818636
OWN - NLM
STAT- MEDLINE
DCOM- 19970501
LR  - 20190826
IS  - 0891-5849 (Print)
IS  - 0891-5849 (Linking)
VI  - 21
IP  - 2
DP  - 1996
TI  - The effect of ascorbate and ubiquinone supplementation on plasma and CSF total 
      antioxidant capacity.
PG  - 211-7
AB  - Free radicals are thought to be involved in the onset of neuronal disturbances 
      such as Alzheimer's disease, Parkinson's disease, and neuronal ceroid 
      lipofuscinosis. It is also assumed that they play a role in cerebral injury 
      caused by ischemia or trauma. Plasma and cerebrospinal fluid (CSF), Total 
      (peroxyl) Radical-trapping Antioxidant Parameter (TRAP), and the known 
      antioxidant components of TRAP, for instance, ascorbic acid, uric acid, protein 
      sulfhydryl groups, tocopherol, and ubiquinol were analyzed and the remaining 
      unidentified fragment was calculated in five healthy volunteers before and after 
      4 weeks of ascorbate and ubiquinone (Q-10) supplementation. In CSF, TRAP was 
      significantly lower than in plasma. The major contributor to plasma's antioxidant 
      capacity was uric acid (UA), whereas in CSF it was ascorbic acid (AA). In CSF, AA 
      concentrations were four times higher than in plasma. Oral supplementation of AA 
      (500 mg/d first 2 weeks, 1,000 mg/d following 2 weeks) and Q-10 (100 mg/d first 2 
      weeks, 300 mg/d following 2 weeks) induced a significant increase in plasma AA 
      and Q-10. Surprisingly, in spite of the high lipophilicity of Q-10, its 
      concentration did not change in CSF. The supplementation of AA increased its 
      concentration in CSF by 28% (p < .05). However, the increase in AA did not result 
      in an increase in CSF TRAP. This indicates that AA had lost one-third of its 
      radical trapping capacity as compared to that in plasma. The facts that AA is the 
      highest contributor to CSF TRAP and its effect on TRAP is concentration dependent 
      could indicate that the peroxyl radical-trapping capacity of CSF is buffered by 
      AA.
FAU - Lonnrot, K
AU  - Lonnrot K
AD  - Department of Biomedical Sciences, University of Tampere, Finland.
FAU - Metsa-Ketela, T
AU  - Metsa-Ketela T
FAU - Molnar, G
AU  - Molnar G
FAU - Ahonen, J P
AU  - Ahonen JP
FAU - Latvala, M
AU  - Latvala M
FAU - Peltola, J
AU  - Peltola J
FAU - Pietila, T
AU  - Pietila T
FAU - Alho, H
AU  - Alho H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Free Radic Biol Med
JT  - Free radical biology & medicine
JID - 8709159
RN  - 0 (Antioxidants)
RN  - 0 (Free Radicals)
RN  - 0 (Sulfhydryl Compounds)
RN  - 1339-63-5 (Ubiquinone)
RN  - 1406-18-4 (Vitamin E)
RN  - 268B43MJ25 (Uric Acid)
RN  - M9NL0C577Y (ubiquinol)
RN  - PQ6CK8PD0R (Ascorbic Acid)
SB  - IM
MH  - Adult
MH  - Antioxidants/*analysis
MH  - Ascorbic Acid/blood/cerebrospinal fluid/*pharmacology
MH  - Free Radicals
MH  - Humans
MH  - Male
MH  - Sulfhydryl Compounds/blood/cerebrospinal fluid
MH  - Ubiquinone/analogs & derivatives/blood/cerebrospinal fluid/*pharmacology
MH  - Uric Acid/blood/cerebrospinal fluid
MH  - Vitamin E/blood/cerebrospinal fluid
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 0891584995022074 [pii]
AID - 10.1016/0891-5849(95)02207-4 [doi]
PST - ppublish
SO  - Free Radic Biol Med. 1996;21(2):211-7. doi: 10.1016/0891-5849(95)02207-4.