PMID- 8818657
OWN - NLM
STAT- MEDLINE
DCOM- 19961112
LR  - 20061115
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 16
IP  - 2
DP  - 1996 Jun
TI  - Clonal origin of trisomy for chromosome 7 in the epithelial compartment of colon 
      neoplasia.
PG  - 106-12
AB  - In this study, we demonstrated the clonal origin of trisomy for chromosome 7 in 
      epithelial cells of colon neoplasia. By using the double-target fluorescence in 
      situ hybridization (FISH) technique in frozen tissue sections that were also 
      immunostained for keratin and vimentin, ratio analysis of FISH signals for 
      chromosomes 7 and 17 could be performed in epithelial (cytokeratin-positive) or 
      stromal (vimentin-positive) areas. The data demonstrated that trisomy for 
      chromosome 7 is found exclusively in the epithelial compartments and not in the 
      stroma of colon adenocarcinoma. We then demonstrated the occurrence of trisomy 
      for chromosome 7 in the different types of epithelial neoplastic cells, i.e., 
      columnar and goblet cells, which were isolated from frozen tissue sections by 
      mechanical disaggregation of colon tissue and mild lysis of the cells while 
      protease activity was inhibited. In these cell suspensions, the columnar cells 
      were detected with an antibody to villin, and the goblet cells were stained for 
      mucin, whereas all cells were subsequently subjected to FISH for chromosome 7. 
      For analysis of neuroendocrine cells, which are present in a very low frequency 
      in colon neoplasia, frozen tissue sections that were immunostained for 
      Chromogranin A could be used. Individual neuroendocrine cells could be 
      distinguished in these thin frozen tissue sections. The presence of trisomy for 
      chromosome 7 in all three different epithelial cell types strengthens our 
      suggestion that this chromosomal aberration is found in the epithelial stem cell 
      compartment of colon neoplasia.
FAU - Herbergs, J
AU  - Herbergs J
AD  - Department of Pathology, University Hospital Maastrict, The Netherlands.
FAU - Arends, J W
AU  - Arends JW
FAU - Bongers, E M
AU  - Bongers EM
FAU - Ramaekers, F C
AU  - Ramaekers FC
FAU - Hopman, A H
AU  - Hopman AH
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (Vimentin)
RN  - 68238-35-7 (Keratins)
SB  - IM
MH  - Adenoma/genetics
MH  - Carcinoma/genetics
MH  - Cell Line
MH  - Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 7
MH  - Clone Cells
MH  - Colonic Neoplasms/*genetics
MH  - Epithelium/chemistry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Keratins/analysis
MH  - *Trisomy
MH  - Vimentin/analysis
EDAT- 1996/06/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1996/06/01 00:00
PHST- 1996/06/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1996/06/01 00:00 [entrez]
AID - 10.1002/(SICI)1098-2264(199606)16:2<106::AID-GCC4>3.0.CO;2-3 [pii]
AID - 10.1002/(SICI)1098-2264(199606)16:2<106::AID-GCC4>3.0.CO;2-3 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1996 Jun;16(2):106-12. doi: 
      10.1002/(SICI)1098-2264(199606)16:2<106::AID-GCC4>3.0.CO;2-3.