PMID- 8818962
OWN - NLM
STAT- MEDLINE
DCOM- 19961204
LR  - 20220110
IS  - 0146-6615 (Print)
IS  - 0146-6615 (Linking)
VI  - 49
IP  - 3
DP  - 1996 Jul
TI  - Genotypes of hepatitis C virus in Nigeria.
PG  - 178-86
AB  - A pilot survey of hepatitis C virus (HCV) infection in Nigeria was carried out on 
      healthy adult blood donors and children of preschool age. Sixteen of 200 (8%) 
      donors were positive for antibodies using a second generation enzyme-linked 
      immunosorbent assay (ELISA) but all of the children were negative. Supplementary 
      testing of the ELISA-positives using a recombinant immunoblot assay (RIBA-2) 
      confirmed the presence of antibody in four and two others were indeterminate. 
      Four of the anti-HCV-positive sera and one found positive by ELISA but which was 
      negative by RIBA-2 were found to be positive for HCV RNA using reverse 
      transcriptase-polymerase chain reaction (RT-PCR) and primers specific for the 5' 
      untranslated region (5'UTR) of the HCV genome. The NS5 and core regions also were 
      amplified and the PCR products from all three regions were sequenced. Sequences 
      from the 5'UTR could be divided into two groups: one group comprised three 
      isolates with greater than 95% sequence identity with published sequences of 
      genotype 1 and the other comprised two isolates with greater than 93% sequence 
      identity with genotype 4. Analysis of three sequences amplified from the NS5 
      region confirmed this assignment to genotypes 1 and 4. Pairwise comparisons of 
      the NS5 region sequences with representatives of 1a, 1b, 1c (for the first group) 
      and 4a-4h (for the second group) show the first group to include subtypes 
      classifiable as 1a and a novel sequence and the second group to include a novel 
      sequence within genotype 4. Sequence analysis of the core region was consistent 
      with this interpretation. These data confirm the presence of at least two major 
      HCV genotypes in Nigeria (genotypes 1 and 4) and we report two novel sequences 
      which have been designated provisionally as genotypes 1d and 4i.
FAU - Oni, A O
AU  - Oni AO
AD  - University Department of Medicine, Royal Free Hospital School of Medicine, 
      London, United Kingdom.
FAU - Harrison, T J
AU  - Harrison TJ
LA  - eng
SI  - GENBANK/L29587
SI  - GENBANK/L29624
SI  - GENBANK/U10235
SI  - GENBANK/U10236
SI  - GENBANK/U10238
SI  - GENBANK/U10239
SI  - GENBANK/U10240
PT  - Journal Article
PL  - United States
TA  - J Med Virol
JT  - Journal of medical virology
JID - 7705876
RN  - 0 (DNA, Viral)
RN  - 0 (Viral Core Proteins)
RN  - 0 (Viral Nonstructural Proteins)
RN  - 0 (nucleocapsid protein, Hepatitis C virus)
RN  - EC 2.7.7.48 (NS-5 protein, hepatitis C virus)
SB  - IM
MH  - Adult
MH  - Base Sequence
MH  - Child, Preschool
MH  - DNA, Viral
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Genotype
MH  - Hepacivirus/classification/*genetics/immunology/isolation & purification
MH  - Hepatitis C/blood/immunology/virology
MH  - Humans
MH  - Molecular Sequence Data
MH  - Nigeria
MH  - Pilot Projects
MH  - Polymerase Chain Reaction
MH  - Sequence Homology, Nucleic Acid
MH  - Viral Core Proteins/genetics
MH  - Viral Nonstructural Proteins/genetics
EDAT- 1996/07/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1996/07/01 00:00
PHST- 1996/07/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1996/07/01 00:00 [entrez]
AID - 10.1002/(SICI)1096-9071(199607)49:3<178::AID-JMV4>3.0.CO;2-1 [pii]
AID - 10.1002/(SICI)1096-9071(199607)49:3<178::AID-JMV4>3.0.CO;2-1 [doi]
PST - ppublish
SO  - J Med Virol. 1996 Jul;49(3):178-86. doi: 
      10.1002/(SICI)1096-9071(199607)49:3<178::AID-JMV4>3.0.CO;2-1.