PMID- 8819277
OWN - NLM
STAT- MEDLINE
DCOM- 19970219
LR  - 20190914
IS  - 0934-0874 (Print)
IS  - 0934-0874 (Linking)
VI  - 9
IP  - 4
DP  - 1996
TI  - Retrograde oxygen persufflation in combination with UW solution enhances adenine 
      nucleotide contents in ischemically damaged rat kidney during cold storage.
PG  - 396-402
AB  - Retrograde oxygen persufflation (ROP) has been reported to be beneficial to 
      kidney preservation. The purpose of this study was to investigate whether use of 
      ROP during cold storage (CS) with Universita of Wisconsin (UW) solution could 
      ameliorate energy metabolism and functional recovery of ischemically injured rat 
      kidneys and, moreover, to study the particular role of adenosine (ADO) in CS with 
      ROP. Kidneys subjected to 30 min of warm ischemia (WI) were preserved for 24 h in 
      4 degrees C UW solution with or without ROP and with or without ADO. Measurements 
      of tissue high-energy phosphate levels showed that reduced total adenine 
      nucleotides (TAN) after 30 min of WI further declined during the subsequent CS. 
      In ROP kidneys, however, TAN were less reduced, suggesting that even during CS, 
      TAN can still be regenerated in the injured kidneys when ROP is combined with UW 
      solution. When UW did not contain ADO, regeneration of TAN by ROP was slightly 
      less than in the case of UW with ADO. This indicates that the supply of molecular 
      oxygen is a significant factor in TAN resynthesis during CS. There was no 
      statistically significant difference in survival rate between the ROP and CS 
      groups, indicating that an improved energy status is not the sole determinant of 
      functional recovery. We conclude that the gaseous oxygen supply provided by ROP 
      during CS in UW solution ameliorates the energy state of ischemically injured rat 
      kidneys and that exogenous ADO from the UW solution contributes to the 
      improvement of energy metabolism to a limited extent.
FAU - Yin, M
AU  - Yin M
AD  - Department of Surgery, University Hospital Maastricht, The Netherlands.
FAU - Booster, M H
AU  - Booster MH
FAU - van der Vusse, G J
AU  - van der Vusse GJ
FAU - Maessen, J G
AU  - Maessen JG
FAU - Buurman, W A
AU  - Buurman WA
FAU - Kootstra, G
AU  - Kootstra G
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Transpl Int
JT  - Transplant international : official journal of the European Society for Organ 
      Transplantation
JID - 8908516
RN  - 0 (Adenine Nucleotides)
RN  - 0 (Insulin)
RN  - 0 (Organ Preservation Solutions)
RN  - 0 (Solutions)
RN  - 0 (University of Wisconsin-lactobionate solution)
RN  - 63CZ7GJN5I (Allopurinol)
RN  - GAN16C9B8O (Glutathione)
RN  - K72T3FS567 (Adenosine)
RN  - N5O3QU595M (Raffinose)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Adenine Nucleotides/*metabolism
MH  - Adenosine/administration & dosage/chemistry/metabolism/pharmacology
MH  - Allopurinol/chemistry/pharmacology
MH  - Animals
MH  - Energy Metabolism/drug effects
MH  - Glutathione/chemistry/pharmacology
MH  - Graft Survival
MH  - *Hypothermia, Induced/adverse effects
MH  - Insulin/chemistry/pharmacology
MH  - Ischemia/metabolism
MH  - Kidney/blood supply/*drug effects/metabolism
MH  - *Kidney Transplantation
MH  - Male
MH  - Organ Preservation/*methods
MH  - *Organ Preservation Solutions
MH  - Oxygen/administration & dosage/*pharmacology
MH  - Raffinose/chemistry/pharmacology
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Solutions/chemistry/*pharmacology
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 10.1007/BF00335702 [doi]
PST - ppublish
SO  - Transpl Int. 1996;9(4):396-402. doi: 10.1007/BF00335702.