PMID- 8821456
OWN - NLM
STAT- MEDLINE
DCOM- 19961030
LR  - 20190116
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 364
IP  - 4
DP  - 1996 Jan 22
TI  - Differential expression and modification of neurofilament triplet proteins during 
      cat cerebellar development.
PG  - 704-17
AB  - Neurofilament (NF) proteins consist of three subunits of different molecular 
      weights defined as NF-H, NF-M, and NF-L. They are typical structures of the 
      neuronal cytoskeleton. Their immunocytochemical distribution during postnatal 
      development of cat cerebellum was studied with several monoclonal and polyclonal 
      antibodies against phosphorylated or unmodified sites. Expression and 
      distribution of the triplet neurofilament proteins changed with maturation. 
      Afferent mossy and climbing fibers in the medullary layer contained NF-M and NF-L 
      already at birth, whereas NF-H appeared later. Within the first three postnatal 
      weeks, all three subunits appeared in mossy and climbing fibers in the internal 
      granular and molecular layers and in the axons of Purkinje cells. Axons of local 
      circuit neurons such as basket cells expressed these proteins at the end of the 
      first month, whereas parallel fibers expressed them last, at the beginning of the 
      third postnatal month. Differential localization was especially observed for 
      NF-H. Depending on phosphorylation, NF-H proteins were found in different axon 
      types in climbing, mossy, and basket fibers or additionally in parallel fibers. A 
      nonphosphorylated NF-H subunit was exclusively located in some Purkinje cells at 
      early developmental stages and in some smaller interneurons later. A novel 
      finding is the presence of a phosphorylation site in the NF-H subunit that is 
      localized in dendrites of Purkinje cells but not in axons. Expression and 
      phosphorylation of the NF-H subunit, especially, is cell-type specific and 
      possibly involved in the adult-type stabilization of the axonal and dendritic 
      cytoskeleton.
FAU - Riederer, B M
AU  - Riederer BM
AD  - Institut d'Anatomie, Universite de Lausanne, Switzerland.
FAU - Porchet, R
AU  - Porchet R
FAU - Marugg, R A
AU  - Marugg RA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Neurofilament Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Cats
MH  - Cerebellum/cytology/*growth & development/*metabolism
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Female
MH  - Immunoblotting
MH  - Immunohistochemistry
MH  - Male
MH  - Neurofilament Proteins/*biosynthesis/metabolism
MH  - Phosphorylation
MH  - Protein Processing, Post-Translational/*physiology
MH  - Purkinje Cells/metabolism/physiology
MH  - Spinal Cord/growth & development/metabolism
EDAT- 1996/01/22 00:00
MHDA- 2000/06/20 09:00
CRDT- 1996/01/22 00:00
PHST- 1996/01/22 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1996/01/22 00:00 [entrez]
AID - 10.1002/(SICI)1096-9861(19960122)364:4<704::AID-CNE8>3.0.CO;2-7 [pii]
AID - 10.1002/(SICI)1096-9861(19960122)364:4<704::AID-CNE8>3.0.CO;2-7 [doi]
PST - ppublish
SO  - J Comp Neurol. 1996 Jan 22;364(4):704-17. doi: 
      10.1002/(SICI)1096-9861(19960122)364:4<704::AID-CNE8>3.0.CO;2-7.