PMID- 8821852
OWN - NLM
STAT- MEDLINE
DCOM- 19961121
LR  - 20041117
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 16
IP  - 1
DP  - 1996 Jan
TI  - Maternal cell contamination in uncultured amniotic fluid.
PG  - 49-54
AB  - The presence of maternal cells in uncultured amniotic fluid may result in error 
      in the interpretation of prenatal tests such as direct DNA analysis and rapid 
      aneuploidy detection by fluorescence in situ hybridization (FISH). Using 
      simultaneous dual colour X and Y FISH, we assessed maternal cell contamination in 
      uncultured amniotic fluids from 500 women carrying male fetuses. The presence of 
      maternal cells was correlated with the amount of blood present in the amniotic 
      fluid as defined by visual examination of the cell pellet after centrifugation. 
      The overall rate of maternal cell contamination in uncultured amniotic fluid as 
      identified using X and Y-specific probes was 21.4 per cent, compared with 0.2 per 
      cent in cultured fluid. Sixteen per cent of slightly bloody and 55 per cent of 
      moderately bloody uncultured fluids had at least 20 per cent maternal cells and 
      were classified as uninformative according to our protocol for rapid aneuploidy 
      detection. Maternal and fetal cells could not be distinguished based on 
      morphological characteristics alone.
FAU - Winsor, E J
AU  - Winsor EJ
AD  - Department of Pathology, Toronto Hospital, Canada.
FAU - Silver, M P
AU  - Silver MP
FAU - Theve, R
AU  - Theve R
FAU - Wright, M
AU  - Wright M
FAU - Ward, B E
AU  - Ward BE
LA  - eng
PT  - Journal Article
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
RN  - 0 (DNA Probes)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Amniotic Fluid/*cytology
MH  - Aneuploidy
MH  - Cell Nucleus/ultrastructure
MH  - DNA/*analysis
MH  - DNA Probes
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Pregnancy
MH  - *Prenatal Diagnosis/statistics & numerical data
MH  - X Chromosome
MH  - Y Chromosome
EDAT- 1996/01/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 10.1002/(SICI)1097-0223(199601)16:1<49::AID-PD808>3.0.CO;2-U [pii]
AID - 10.1002/(SICI)1097-0223(199601)16:1<49::AID-PD808>3.0.CO;2-U [doi]
PST - ppublish
SO  - Prenat Diagn. 1996 Jan;16(1):49-54. doi: 
      10.1002/(SICI)1097-0223(199601)16:1<49::AID-PD808>3.0.CO;2-U.