PMID- 8822211
OWN - NLM
STAT- MEDLINE
DCOM- 19970325
LR  - 20150728
IS  - 1044-9523 (Print)
IS  - 1044-9523 (Linking)
VI  - 7
IP  - 2
DP  - 1996 Feb
TI  - Cloning of a novel nucleolar guanosine 5'-triphosphate binding protein 
      autoantigen from a breast tumor.
PG  - 271-80
AB  - A cDNA clone encoding an immunoreactive autoantigen (Ngp-1) was isolated by 
      screening lambda gt11 human ductal breast tumor expression libraries with 
      autologous patient serum. The complete 2.3-kb nucleotide sequence of the cDNA was 
      found to contain an open reading frame that could encode a protein of 731 amino 
      acids. The predicted amino acid sequence contains a high concentration of charged 
      amino acids in the carboxy terminal quarter of the molecule, three guanosine 
      5'-triphosphate (GTP)-binding protein motifs, and a consensus nuclear 
      localization signal. The arrangement and spacing of the GTP-binding protein 
      motifs indicate that Ngp-1 belongs to a newly described subfamily of GTPases. 
      Except for the consensus motifs, neither nucleotide sequence, nor the predicted 
      amino acid sequence of the Ngp-1 cDNA showed the slightest homology to any 
      vertebrate gene product sequence listed in the databases. Northern blot analysis 
      showed the 2.3-kb transcript to be ubiquitously expressed at relatively low 
      levels in all human tissues tested, with the highest level of expression in the 
      testes. Immunohistochemical analysis of tissue sections with affinity-purified 
      antiserum raised against a recombinant Ngp-1 protein revealed that the antigen 
      was exclusively localized to the nucleolus and nucleolar organizer regions in all 
      cell types analyzed.
FAU - Racevskis, J
AU  - Racevskis J
AD  - Department of Oncology, Montefiore and Albert Einstein Cancer Center, Bronx, New 
      York 10467, USA.
FAU - Dill, A
AU  - Dill A
FAU - Stockert, R
AU  - Stockert R
FAU - Fineberg, S A
AU  - Fineberg SA
LA  - eng
SI  - GENBANK/L05425
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Cell Growth Differ
JT  - Cell growth & differentiation : the molecular biology journal of the American 
      Association for Cancer Research
JID - 9100024
RN  - 0 (Autoantibodies)
RN  - 0 (Autoantigens)
RN  - 0 (DNA, Complementary)
RN  - 0 (NGP-1 antigen, human)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Autoantibodies
MH  - Autoantigens/*genetics
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Blotting, Southern
MH  - Blotting, Western
MH  - Breast Neoplasms/*chemistry
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Female
MH  - GTP-Binding Proteins/*genetics/immunology
MH  - Gene Library
MH  - Genetic Testing
MH  - Humans
MH  - Immunohistochemistry
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/*genetics/immunology
MH  - RNA, Messenger/analysis
MH  - Recombinant Proteins/genetics
MH  - Sequence Analysis, DNA
EDAT- 1996/02/01 00:00
MHDA- 1996/02/01 00:01
CRDT- 1996/02/01 00:00
PHST- 1996/02/01 00:00 [pubmed]
PHST- 1996/02/01 00:01 [medline]
PHST- 1996/02/01 00:00 [entrez]
PST - ppublish
SO  - Cell Growth Differ. 1996 Feb;7(2):271-80.