PMID- 8822920
OWN - NLM
STAT- MEDLINE
DCOM- 19961024
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 88
IP  - 6
DP  - 1996 Sep 15
TI  - Expression of Fas/CD95 and Bcl-2 by primitive hematopoietic progenitors freshly 
      isolated from human fetal liver.
PG  - 2013-25
AB  - The cell-surface expression and the functional status of the CD95/Fas antigen on 
      primitive hematopoietic progenitors (PHPs) freshly isolated from human fetal 
      liver (FL) were studied. PHPs were phenotypically defined as CD34++ CD38 -/+ 
      cells. The most immature subfractions of PHPs, CD34++CD38- and CD34+2CD38+ FL 
      cells, expressed CD95, whereas the more mature CD34++CD38++ and CD34+CD38++2 FL 
      cells displayed low CD95 expression. Combinations of cytokines, such as kit 
      ligand (KL) + interleukin-3 or KL + granulocyte-macrophage colony-stimulating 
      factor (GM-CSF) upregulated the expression of CD95 on PHPs upon in vitro culture. 
      Tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) further 
      increased the CD95 expression induced by KL+GM-CSF. The hematopoietic potential 
      of sorted CD34++lineage (lin)- CD95+ versus CD34++ lin-CD95-FL cells was compared 
      by colony-forming unit-culture (CFU-C) assays performed in serum-deprived medium. 
      Lin+ cells were composed of erythrocytes, monocytes, T cells, B cells, and 
      natural killer cells. Our results indicated that both CD95- and CD95+ subsets 
      contained pluripotent progenitors, generating myeloid and erythroid progenitors. 
      The functional status of CD95 and the effects of TNF-alpha and IFN-gamma, 
      cytokines known to induce CD95-mediated apoptosis, were analyzed by incubation of 
      PHPs in the presence of anti-CD95 monoclonal antibodies (MoAbs). The effect of 
      anti-CD95 MoAbs was measured by viable cell counting, flow cytometry, and CFU-C 
      assays. A decrease of CFU-C numbers was observed in the presence of anti-CD95 
      MoAbs and TNF-alpha and/or IFN-gamma. However, whereas growth factor deprivation 
      induced apoptosis of PHPs, cross-linking of CD95 did not lead to apoptosis of 
      PHPs measured by flow cytometry and viable cell counting. The correlation of 
      increased intracytoplasmic levels of bcl-2 with high levels of cell-surface CD34 
      and the presence of CD95 on fresh FL cells suggests that bcl-2 may be involved in 
      protecting against CD95-mediated apoptosis of FL PHPs.
FAU - Barcena, A
AU  - Barcena A
AD  - Ingenex, Inc., Menlo Park, CA, USA.
FAU - Park, S W
AU  - Park SW
FAU - Banapour, B
AU  - Banapour B
FAU - Muench, M O
AU  - Muench MO
FAU - Mechetner, E
AU  - Mechetner E
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, CD34)
RN  - 0 (Interleukin-3)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (Stem Cell Factor)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (fas Receptor)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Antibodies, Monoclonal/immunology
MH  - Antigens, CD34/analysis
MH  - Cell Separation
MH  - Erythroid Precursor Cells/cytology
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Hematopoietic Stem Cells/*cytology
MH  - Humans
MH  - Immunophenotyping
MH  - Interferon-gamma/pharmacology
MH  - Interleukin-3/pharmacology
MH  - Liver/*embryology
MH  - Lymphocytes/cytology
MH  - Proto-Oncogene Proteins/*metabolism
MH  - Proto-Oncogene Proteins c-bcl-2
MH  - Stem Cell Factor/pharmacology
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - Up-Regulation
MH  - fas Receptor/*metabolism
EDAT- 1996/09/15 00:00
MHDA- 1996/09/15 00:01
CRDT- 1996/09/15 00:00
PHST- 1996/09/15 00:00 [pubmed]
PHST- 1996/09/15 00:01 [medline]
PHST- 1996/09/15 00:00 [entrez]
AID - S0006-4971(20)62400-4 [pii]
PST - ppublish
SO  - Blood. 1996 Sep 15;88(6):2013-25.