PMID- 8823932
OWN - NLM
STAT- MEDLINE
DCOM- 19961203
LR  - 20190920
IS  - 1011-1344 (Print)
IS  - 1011-1344 (Linking)
VI  - 35
IP  - 1-2
DP  - 1996 Aug
TI  - Interaction of guanosine 5'-triphosphate binding protein Gq from Sepia 
      officinalis with illuminated rhodopsin bound to concanavalin A.
PG  - 25-31
AB  - The heterotrimeric guanosine 5'-triphosphate (GTP)-binding protein Gq was 
      suggested to couple the light receptor rhodopsin with the effector phospholipase 
      C in visual cells of invertebrates. We indirectly linked Gq from Sepia 
      officinalis to a concanavalin A-sepharose column via rhodopsin. Rhodopsin had 
      been solubilized previously with 10 mM n-dodecyl-beta-maltoside from the purified 
      photosensory membrane under illumination. All three subunits of the Gq were 
      released almost pure by elution with 100 microM GTP. The alpha and beta subunits 
      were identified by specific antipeptide antisera. The alpha subunit has a 
      relative molecular mass of 46 kDa, and the beta subunit of 35 kDa. The gamma 
      subunit corresponds to a 9 kDa polypeptide owing to the molecular mass, which is 
      similar to the G gamma subunit of squid. The use of specific antibodies shows 
      that neither actin nor G-protein related to transducin were in the fractions 
      eluted with GTP or alpha-methyl mannoside. We demonstrate that all three subunits 
      of Gq were associated with rhodopsin of invertebrates. Such use of a lectin 
      column might be useful for further investigations of the interaction of rhodopsin 
      and Gq.
FAU - von der Heide, W
AU  - von der Heide W
AD  - Institut fur Biologie II (Zoologie), Rheinisch-Westfalische Technische Hochschule 
      Aachen, Germany.
FAU - Casaretto, M
AU  - Casaretto M
FAU - Rack, M
AU  - Rack M
FAU - Stieve, H
AU  - Stieve H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - J Photochem Photobiol B
JT  - Journal of photochemistry and photobiology. B, Biology
JID - 8804966
RN  - 0 (Mannosides)
RN  - 0 (Methylmannosides)
RN  - 11028-71-0 (Concanavalin A)
RN  - 51023-63-3 (methylmannoside)
RN  - 9009-81-8 (Rhodopsin)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Chromatography, Affinity
MH  - Concanavalin A/*metabolism
MH  - GTP-Binding Proteins/chemistry/isolation & purification/*metabolism
MH  - Mannosides/metabolism
MH  - Methylmannosides
MH  - Molecular Sequence Data
MH  - Mollusca/*metabolism
MH  - Rhodopsin/*metabolism
EDAT- 1996/08/01 00:00
MHDA- 1996/08/01 00:01
CRDT- 1996/08/01 00:00
PHST- 1996/08/01 00:00 [pubmed]
PHST- 1996/08/01 00:01 [medline]
PHST- 1996/08/01 00:00 [entrez]
AID - 1011-1344(96)07305-8 [pii]
AID - 10.1016/1011-1344(96)07305-8 [doi]
PST - ppublish
SO  - J Photochem Photobiol B. 1996 Aug;35(1-2):25-31. doi: 
      10.1016/1011-1344(96)07305-8.