PMID- 8824722
OWN - NLM
STAT- MEDLINE
DCOM- 19961122
LR  - 20161123
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 15
IP  - 1
DP  - 1996 Jan
TI  - Chromosome microdissection in leukemia: a powerful tool for the analysis of 
      complex chromosomal rearrangements.
PG  - 26-33
AB  - In many human cancers the presence of marker chromosomes or unbalanced 
      translocations prevents complete karyotypic analysis. Chromosome microdissection 
      has become an increasingly important method for assessing chromosome 
      rearrangements. However, most studies using chromosome microdissection have been 
      carried out on established cancer cell lines that provide an unlimited supply of 
      abnormal metaphase cells. We have routinely performed microdissection of as few 
      as three marker chromosome copies from short-term cultures of acute myeloid 
      leukemias, followed by in vitro DNA amplification and fluorescence in situ 
      hybridization (FISH) to normal metaphase spreads. Using this type of "reverse 
      chromosome painting," we were able to characterize precisely the chromosomal 
      constitution of each marker chromosome in the samples, confirming the diagnostic 
      usefulness of microdissection in cancer cytogenetics. In addition, in one 
      leukemia with atypical cytological features, microdissection enabled us to detect 
      a novel retinoic acid receptor alpha gene rearrangement.
FAU - Jonveaux, P
AU  - Jonveaux P
AD  - Unite INSERM U.301, Institut de Genetique Moleculaire, Paris, France.
FAU - Le Coniat, M
AU  - Le Coniat M
FAU - Derre, J
AU  - Derre J
FAU - Flexor, M A
AU  - Flexor MA
FAU - Daniel, M T
AU  - Daniel MT
FAU - Berger, R
AU  - Berger R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (DNA Primers)
RN  - 0 (RARA protein, human)
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Retinoic Acid Receptor alpha)
SB  - IM
MH  - Acute Disease
MH  - Aged
MH  - *Chromosome Aberrations
MH  - *Chromosome Disorders
MH  - DNA Primers
MH  - Female
MH  - Gene Rearrangement
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leukemia/genetics
MH  - Lymphoma, B-Cell/genetics
MH  - Male
MH  - Middle Aged
MH  - Receptors, Retinoic Acid/genetics
MH  - Retinoic Acid Receptor alpha
MH  - *Translocation, Genetic
MH  - Tumor Cells, Cultured
EDAT- 1996/01/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 10.1002/(SICI)1098-2264(199601)15:1<26::AID-GCC4>3.0.CO;2-6 [pii]
AID - 10.1002/(SICI)1098-2264(199601)15:1<26::AID-GCC4>3.0.CO;2-6 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1996 Jan;15(1):26-33. doi: 
      10.1002/(SICI)1098-2264(199601)15:1<26::AID-GCC4>3.0.CO;2-6.