PMID- 8825634
OWN - NLM
STAT- MEDLINE
DCOM- 19970206
LR  - 20190905
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 30
IP  - 3
DP  - 1995 Dec 10
TI  - Identification of neurofibromatosis 1 (NF1) homologous loci by direct sequencing, 
      fluorescence in situ hybridization, and PCR amplification of somatic cell 
      hybrids.
PG  - 476-85
AB  - Using fluorescence in situ hybridization (FISH), we have identified seven 
      NF1-related loci, two separate loci on chromosome 2, at bands 2q21 and 2q33-q34, 
      and one locus each on five other chromosomes at bands 14q11.2, 15q11.2, 18p11.2, 
      21q11.2-q21, and 22q11.2. Application of PCR using NF1 primer pairs and genomic 
      DNA from somatic cell hybrids confirmed the above loci, identified additional 
      loci on chromosomes 12 and 15, and showed that the various loci do not share 
      homology beyond NF1 exon 27b. Sequenced PCR products representing segments 
      corresponding to NF1 exons from these loci demonstrated greater than 95% sequence 
      identity with the NF1 locus. We used sequence differences between bona fide NF1 
      and NF1-homologous loci to strategically design primer sets to specifically 
      amplify 30 of 36 exons within the 5' end of the NF1 gene. These developments have 
      facilitated mutation analysis at the NF1 locus using genomic DNA as template.
FAU - Purandare, S M
AU  - Purandare SM
AD  - Department of Pediatrics, University of Utah, Salt Lake City 84112, USA.
FAU - Huntsman Breidenbach, H
AU  - Huntsman Breidenbach H
FAU - Li, Y
AU  - Li Y
FAU - Zhu, X L
AU  - Zhu XL
FAU - Sawada, S
AU  - Sawada S
FAU - Neil, S M
AU  - Neil SM
FAU - Brothman, A
AU  - Brothman A
FAU - White, R
AU  - White R
FAU - Cawthon, R
AU  - Cawthon R
FAU - Viskochil, D
AU  - Viskochil D
LA  - eng
SI  - GENBANK/U35684
SI  - GENBANK/U35685
SI  - GENBANK/U35686
SI  - GENBANK/U35687
SI  - GENBANK/U35688
SI  - GENBANK/U35689
SI  - GENBANK/U35690
SI  - GENBANK/U35691
SI  - GENBANK/U35692
SI  - GENBANK/U35693
SI  - GENBANK/U35694
SI  - GENBANK/U35695
SI  - GENBANK/U35696
SI  - GENBANK/U35697
SI  - GENBANK/U35698
SI  - GENBANK/U35699
GR  - K08 NS01492/NS/NINDS NIH HHS/United States
GR  - R55CA57511-01/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (DNA Primers)
RN  - 0 (Neurofibromin 1)
RN  - 0 (Proteins)
SB  - IM
MH  - Animals
MH  - Base Composition
MH  - Base Sequence
MH  - *Chromosome Mapping
MH  - Chromosomes, Human, Pair 15/*genetics
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - DNA Primers
MH  - Humans
MH  - Hybrid Cells
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Neurofibromin 1
MH  - Polymerase Chain Reaction
MH  - Proteins/*genetics
EDAT- 1995/12/10 00:00
MHDA- 1995/12/10 00:01
CRDT- 1995/12/10 00:00
PHST- 1995/12/10 00:00 [pubmed]
PHST- 1995/12/10 00:01 [medline]
PHST- 1995/12/10 00:00 [entrez]
AID - S0888754385712682 [pii]
AID - 10.1006/geno.1995.1268 [doi]
PST - ppublish
SO  - Genomics. 1995 Dec 10;30(3):476-85. doi: 10.1006/geno.1995.1268.