PMID- 8826096
OWN - NLM
STAT- MEDLINE
DCOM- 19961204
LR  - 20031114
IS  - 0098-6127 (Print)
IS  - 0098-6127 (Linking)
VI  - 21
IP  - 5
DP  - 1994
TI  - Ubiquitin expression in atherosclerotic lesions of wistar fatty and wistar lean 
      rats.
PG  - 256-70
AB  - To clarify whether ubiquitin is expressed in atherosclerotic lesions, and, if so, 
      the expression is influenced by diabetes mellitus, we examined atherosclerotic 
      (AS) lesions from Wistar fatty (WF) and Wistar lean (WL) rats 
      immunohistochemically using an antibody against ubiquitin (AUb). Ten-week-old 
      male WF and WL rats were treated to cannulize a silicon tube from the left 
      carotid artery (LCA) to the descending aorta under chloral hydrate anesthesia and 
      the tube was fixed. Age-matched WF and WL rats without cannulization were served 
      as controls. Eight weeks after operation, 1 ml of 0.1% Evans blue solution was 
      injected to all rats from the tail vein. 15 min latter, the aortae were removed, 
      fixed with 4% paraformaldehyde and embedded in paraffin. Immunohistochemical 
      staining with AUb by the ABC method, hematoxylin and eosin (HE) and 
      elastica-Goldner (EG) stains were performed. In the cannulized group, focal areas 
      of the luminal surface of the aorta were stained blue with Evans blue and these 
      areas were microscopically confirmed as AS lesions in all WF and WL rats. In the 
      control group, no Evans blue staining or AS lesions were observed. The 
      destruction of the internal elastic lamina in AS lesions were seen with EG stain 
      in the cannulized aorta of both WF and WL rats. No significant difference of the 
      area ratio of intima/media was present between WF and WL rats in the cannulized 
      group. Ubiquitin immunoreactivity was observed in the nucleus and cytoplasm of 
      cells in AS lesions of both WF and WL rats. The present study suggests that 
      ubiquitin plays a role in the formation of AS, and the condition of diabetes 
      mellitus has little influence on ubiquitin expression and AS formation in this 
      experimental model.
FAU - Igarashi, M
AU  - Igarashi M
AD  - Third Department of Internal Medicine, Yamagata University School of Medicine, 
      Japan.
FAU - Kato, T
AU  - Kato T
FAU - Ohnuma, H
AU  - Ohnuma H
FAU - Morita, Y
AU  - Morita Y
FAU - Kawanami, T
AU  - Kawanami T
FAU - Sasaki, H
AU  - Sasaki H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Artery
JT  - Artery
JID - 7508494
RN  - 0 (Ubiquitins)
SB  - IM
MH  - Animals
MH  - Arteriosclerosis/*metabolism/pathology
MH  - Diabetes Mellitus, Experimental/*metabolism/pathology
MH  - Female
MH  - Immunohistochemistry
MH  - Male
MH  - Rats
MH  - Rats, Wistar
MH  - Ubiquitins/*biosynthesis
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
PST - ppublish
SO  - Artery. 1994;21(5):256-70.