PMID- 8830470
OWN - NLM
STAT- MEDLINE
DCOM- 19961016
LR  - 20190622
IS  - 0065-2598 (Print)
IS  - 0065-2598 (Linking)
VI  - 380
DP  - 1995
TI  - Isolation and experimental oral transmission in pigs of a porcine reproductive 
      and respiratory syndrome virus isolate.
PG  - 139-44
AB  - A virus inducing a cytopathic effect on porcine alveolar macrophages was isolated 
      from the lungs of a pig with respiratory problems and lesions of proliferative 
      and necrotizing pneumonia. The isolate was found to react with porcine 
      reproductive and respiratory syndrome virus (PRRSV) monoclonal antibodies by 
      indirect immunofluorescence and was designated LHVA-93-3. The virus could also be 
      propagated on the MARC-145 cell line. The LHVA-93-3 macrophage-passaged isolate 
      was inoculated orally or intranasally in four-week-old specific pathogen-free 
      pigs. Histologically, focal to multifocal lesions of proliferative, necrotizing 
      and interstitial pneumonia could be observed in the lungs of pigs inoculated 
      orally or intranasally, 6 and 10 days post-inoculation. Virus could be reisolated 
      from essentially the same tissues including serum following both routes of 
      infection. The distribution of PRRSV antigens in fixed tissues as determined by 
      immunogold silver staining (IGSS) was similar in orally or intranasally 
      inoculated pigs. The results of this experimental transmission study indicate 
      that pigs may become infected by PRRSV following oral as well as intranasal 
      exposure.
FAU - Magar, R
AU  - Magar R
AD  - Laboratoire d'Hygiene Veterinaire et Alimentaire, Agriculture Canada, 
      Saint-Hyacinthe, Quebec, Canada.
FAU - Robinson, Y
AU  - Robinson Y
FAU - Dubuc, C
AU  - Dubuc C
FAU - Larochelle, R
AU  - Larochelle R
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Adv Exp Med Biol
JT  - Advances in experimental medicine and biology
JID - 0121103
SB  - IM
MH  - Animals
MH  - Female
MH  - Fluorescent Antibody Technique, Indirect
MH  - Infertility, Female/*veterinary/virology
MH  - Lung/pathology/virology
MH  - Microscopy, Immunoelectron
MH  - Mouth/*virology
MH  - Nasal Cavity/virology
MH  - Peyer's Patches/pathology/virology
MH  - Respiratory Tract Infections/transmission/*veterinary/virology
MH  - Swine
MH  - *Swine Diseases
MH  - Syndrome
MH  - Togaviridae/*isolation & purification/pathogenicity
MH  - Togaviridae Infections/transmission/*veterinary/virology
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - 10.1007/978-1-4615-1899-0_23 [doi]
PST - ppublish
SO  - Adv Exp Med Biol. 1995;380:139-44. doi: 10.1007/978-1-4615-1899-0_23.