PMID- 8830492
OWN - NLM
STAT- MEDLINE
DCOM- 19961016
LR  - 20190622
IS  - 0065-2598 (Print)
IS  - 0065-2598 (Linking)
VI  - 380
DP  - 1995
TI  - Structural gene analysis of a Quebec reference strain or porcine reproductive and 
      respiratory syndrome virus (PRRSV).
PG  - 277-81
AB  - The 3' end genomic region of a Quebec PRRSV reference strain (IAF-exp91), 
      propagated in porcine alveolar macrophages (PAM), was sequenced and compared to 
      the prototype European strain, the Lelystad virus (LV). The sequence, which 
      represents the 3'-terminal 2834 nucleotides, encompassed 5 ORFs corresponding to 
      ORFs 3 to 7 of LV. Extensive genomic variations resulting from an important rate 
      of nucleotide additions, substitutions, and deletions were demonstrated between 
      the two viruses. Indeed, the two corresponding sequences displayed a total of 66% 
      and 63% identity at the nucleotide and amino acid levels, respectively. The 
      predicted products of ORFs 5, 3, and 7, showed the highest rate of amino acid 
      variations with percentages of identity of 52, 54, and 59, respectively. Sequence 
      analysis of an additional Quebec strain that could be propagated in a continuous 
      cell line (MARC-145), suggested that Quebec PRRSV strains belong to a genotype 
      distinct from that of LV, thus confirming previous serological results which 
      allowed to divide PRRSV isolates into two distinct antigenic subgroups (U.S. and 
      European). Six viral major polypeptides with apparent M(rs) of 14.5K, 15K, 19K, 
      24.5K, 29K, and 42K could be identified from lysates of viral infected cells, of 
      which the 15, 19 and 24.5K species seemed to be structural. In vitro translation 
      products of ORFs 7 and 6 comigrated with the 15 and 19 K viral proteins, whereas 
      that of ORF 5 may be associated to the 24.5K when translated in presence of 
      microsomes. Consequently, it is likely that ORFs 7 to 5, encode the three major 
      structural proteins.
FAU - Mardassi, H
AU  - Mardassi H
AD  - Centre De Recherche En Virologie, Institut Armand-Frappier, Universite Du Quebec, 
      Laval, Canada.
FAU - Mounir, S
AU  - Mounir S
FAU - Dea, S
AU  - Dea S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Adv Exp Med Biol
JT  - Advances in experimental medicine and biology
JID - 0121103
RN  - 0 (Sulfur Radioisotopes)
RN  - 0 (Viral Proteins)
RN  - 0 (Viral Structural Proteins)
RN  - AE28F7PNPL (Methionine)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Caliciviridae/classification/*genetics/metabolism
MH  - Caliciviridae Infections/*veterinary/virology
MH  - Consensus Sequence
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Europe
MH  - *Genes, Viral
MH  - Methionine/metabolism
MH  - Molecular Weight
MH  - Open Reading Frames
MH  - Protein Biosynthesis
MH  - Quebec
MH  - Respiratory Tract Infections/*veterinary/virology
MH  - Sulfur Radioisotopes
MH  - Swine
MH  - *Swine Diseases
MH  - Syndrome
MH  - Viral Proteins/*biosynthesis/genetics
MH  - Viral Structural Proteins/*genetics
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - 10.1007/978-1-4615-1899-0_44 [doi]
PST - ppublish
SO  - Adv Exp Med Biol. 1995;380:277-81. doi: 10.1007/978-1-4615-1899-0_44.