PMID- 8830725
OWN - NLM
STAT- MEDLINE
DCOM- 19961104
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 90
IP  - 2
DP  - 1996 Sep
TI  - Alteration of numerical chromosomal aberrations during progression of colorectal 
      tumors revealed by a combined fluorescence in situ hybridization and DNA ploidy 
      analysis of intratumoral heterogeneity.
PG  - 146-53
AB  - To trace the sequence of numerical chromosomal aberrations during tumor 
      progression of colorectal tumors, we studied intratumoral heterogeneity of 
      chromosomal copy number by a combined fluorescence in situ hybridization (FISH) 
      and ploidy analysis. We used six formalin-fixed paraffin-embedded tumors of which 
      the mucosal lesions were preserved. Nuclear suspensions were made from the tumor 
      tissues that were scraped from several small regions in 100 micron thick 
      sections. Copy number of chromosomes 1, 7, 17, and 18 were examined by FISH with 
      centromeric repetitive probes. DNA ploidy was monitored by cytofluorometry, and 
      was correlated to the chromosomal copy number on the smear slides of identical 
      nuclear suspension from each tumor portion. All the tumors examined included the 
      DNA-diploid regions in the mucosa, where cancer cells commonly showed monosomy 18 
      and/or trisomy 7. These chromosomal changes may be quite common early events 
      before the occurrence of DNA-aneuploidy in the development of colorectal tumors. 
      Three out of the 6 tumors included near-tetraploid (3.6-4.1C) cells in deeper 
      invasive regions. Chromosomal constitution of DNA-aneuploid cells was suggested 
      to derive from that of DNA-diploid cells through ploidy duplication with or 
      without additional loss or gain of chromosomes.
FAU - Katsura, K
AU  - Katsura K
AD  - Department of Pathology, Kyoto Prefectural University of Medicine, Japan.
FAU - Sugihara, H
AU  - Sugihara H
FAU - Nakai, S
AU  - Nakai S
FAU - Fujita, S
AU  - Fujita S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Centromere
MH  - *Chromosome Aberrations
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 1
MH  - Chromosomes, Human, Pair 17
MH  - Chromosomes, Human, Pair 18
MH  - Chromosomes, Human, Pair 7
MH  - Chromosomes, Human, Pair 8
MH  - Colonic Neoplasms/genetics/pathology/surgery
MH  - Colorectal Neoplasms/*genetics/pathology
MH  - DNA, Neoplasm/*analysis
MH  - Diploidy
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Intestinal Mucosa/pathology
MH  - Monosomy
MH  - *Ploidies
MH  - Rectal Neoplasms/genetics/pathology/surgery
MH  - Sigmoid Neoplasms/genetics/pathology/surgery
MH  - Trisomy
EDAT- 1996/09/01 00:00
MHDA- 1996/09/01 00:01
CRDT- 1996/09/01 00:00
PHST- 1996/09/01 00:00 [pubmed]
PHST- 1996/09/01 00:01 [medline]
PHST- 1996/09/01 00:00 [entrez]
AID - S0165460896000660 [pii]
AID - 10.1016/s0165-4608(96)00066-0 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1996 Sep;90(2):146-53. doi: 
      10.1016/s0165-4608(96)00066-0.