PMID- 8831899
OWN - NLM
STAT- MEDLINE
DCOM- 19970206
LR  - 20200418
IS  - 1046-7408 (Print)
IS  - 1600-0897 (Electronic)
IS  - 1046-7408 (Linking)
VI  - 36
IP  - 1
DP  - 1996 Jul
TI  - Maternal antibodies protect immunoglobulin deficient neonatal mice from mouse 
      hepatitis virus (MHV)-associated wasting syndrome.
PG  - 33-9
AB  - PROBLEM: Neonatal mice nursed by dams lacking immunoglobulins (Igs) may often 
      suffer from lethal runting if raised under conventional conditions. The present 
      study was performed in order to clarify a) the cause of the wasting syndrome and 
      b) the protective role of antigen-specific milk antibodies. METHOD: Ig-deficient 
      mouse embryos in a conventional environment were embryo-transferred to specified 
      pathogen free (SPF) dams. Neonatal growth, mortality, and health status of mice 
      from both environments was recorded. Suspected presence of mouse hepatitis virus 
      (MHV) was tested by RT-PCR. Protective effects on neonatal mortality of milk 
      containing different titers of anti-MHV antibodies were investigated in 
      cross-fostering experiments. RESULTS: The SPF colony of Ig-deficient mice 
      exhibited no breeding problems, whereas Ig-deficient neonates in the conventional 
      environment suffered from lethal wasting syndrome. Serological screening of the 
      mice kept in the two environments revealed that mice in the conventional room had 
      high titers of antibodies against mouse hepatitis virus. Presence of MHV in 
      runting neonates was confirmed by pathological examinations and RT-nested-PCR 
      using MHV genome specific primers. Milk containing high titers of anti-MHV 
      antibodies, when provided for 8 days or more, completely prevented Ig-deficient 
      neonates from developing wasting syndrome in the conventional environment. 
      CONCLUSION: These findings show that the neonatal wasting syndrome is associated 
      with the presence of MHV and that neonates are efficiently protected by 
      MHV-specific antibodies in the milk.
FAU - Gustafsson, E
AU  - Gustafsson E
AD  - Department of Animal Development and Genetics, Uppsala University, Sweden.
FAU - Blomqvist, G
AU  - Blomqvist G
FAU - Bellman, A
AU  - Bellman A
FAU - Holmdahl, R
AU  - Holmdahl R
FAU - Mattsson, A
AU  - Mattsson A
FAU - Mattsson, R
AU  - Mattsson R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Am J Reprod Immunol
JT  - American journal of reproductive immunology (New York, N.Y. : 1989)
JID - 8912860
RN  - 0 (Antibodies, Viral)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Immunoglobulins)
RN  - 0 (beta 2-Microglobulin)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Antibodies, Viral/*immunology
MH  - Female
MH  - Hepatitis, Viral, Animal/etiology
MH  - Histocompatibility Antigens Class II/analysis
MH  - Immunoglobulins/*deficiency
MH  - Immunologic Deficiency Syndromes/immunology
MH  - Lactation/*immunology
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Murine hepatitis virus/*immunology/*pathogenicity
MH  - Pregnancy
MH  - Wasting Syndrome/etiology/*prevention & control/*virology
MH  - beta 2-Microglobulin/deficiency
PMC - PMC7159460
EDAT- 1996/07/01 00:00
MHDA- 1996/07/01 00:01
PMCR- 2020/04/15
CRDT- 1996/07/01 00:00
PHST- 1996/07/01 00:00 [pubmed]
PHST- 1996/07/01 00:01 [medline]
PHST- 1996/07/01 00:00 [entrez]
PHST- 2020/04/15 00:00 [pmc-release]
AID - AJI136 [pii]
AID - 10.1111/j.1600-0897.1996.tb00136.x [doi]
PST - ppublish
SO  - Am J Reprod Immunol. 1996 Jul;36(1):33-9. doi: 
      10.1111/j.1600-0897.1996.tb00136.x.