PMID- 8831930
OWN - NLM
STAT- MEDLINE
DCOM- 19970224
LR  - 20190718
IS  - 0021-9150 (Print)
IS  - 0021-9150 (Linking)
VI  - 125
IP  - 1
DP  - 1996 Aug 23
TI  - Detection and localization of tumor necrosis factor-alpha in WHHL rabbit 
      arteries.
PG  - 81-9
AB  - Tumor necrosis factor-alpha (TNF-alpha) is known to be a secretory product of 
      activated macrophages. TNF-alpha activates endothelial cells, stimulates 
      angiogenesis and induces proliferation of smooth muscle cells (SMCs). Therefore, 
      TNF-alpha has been suggested to be actively involved in the inflammatory events 
      associated with atherosclerosis. Previous ultrastructural and immunocytochemical 
      studies have shown that macrophages as well as SMCs are constituents of 
      atherosclerotic lesions in the Watanabe heritable hyperlipidemic (WHHL) rabbit. 
      Recently, we have shown that TNF-alpha mRNA levels in aorta of 18-month-old WHHL 
      rabbits were significantly higher than that of 6-month-old WHHL rabbits and New 
      Zealand White (NZW) rabbits by quantitative RT-PCR [1]. However, it remains 
      unclear as to the cell type(s) responsible for the increased TNF-alpha mRNA 
      levels in atherosclerotic lesions. In this study, we provided evidence showing 
      the expression of the TNF-alpha gene in the medial SMCs as well as cells of 
      intimal lesions in arteries of WHHL rabbits by in situ transcription (IST). 
      TNF-alpha protein was also detected in the cytoplasm of the intimal and medial 
      SMCs and macrophages by immunocytochemistry using a monoclonal antibody against 
      rabbit TNF-alpha. In contrast, the expression of TNF-alpha mRNA and protein can 
      not be detected in the arteries from healthy New Zealand White (NZW) rabbits. Our 
      results suggest that the expression of TNF-alpha in both intimal and medial SMCs 
      and macrophages is associated with the progression of atherosclerosis.
FAU - Lei, X
AU  - Lei X
AD  - Department of Pathology, University of Texas Medical School, Houston 77030, USA.
FAU - Buja, L M
AU  - Buja LM
LA  - eng
PT  - Journal Article
PL  - Ireland
TA  - Atherosclerosis
JT  - Atherosclerosis
JID - 0242543
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Aorta/*metabolism
MH  - Aorta, Thoracic/metabolism
MH  - Arteriosclerosis/*metabolism
MH  - Base Sequence
MH  - Disease Models, Animal
MH  - Female
MH  - Hyperlipoproteinemia Type II/*metabolism
MH  - Immunoblotting
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - RNA, Messenger/analysis
MH  - Rabbits
MH  - Tumor Necrosis Factor-alpha/*analysis
EDAT- 1996/08/23 00:00
MHDA- 1996/08/23 00:01
CRDT- 1996/08/23 00:00
PHST- 1996/08/23 00:00 [pubmed]
PHST- 1996/08/23 00:01 [medline]
PHST- 1996/08/23 00:00 [entrez]
AID - 0021-9150(96)05863-7 [pii]
AID - 10.1016/0021-9150(96)05863-7 [doi]
PST - ppublish
SO  - Atherosclerosis. 1996 Aug 23;125(1):81-9. doi: 10.1016/0021-9150(96)05863-7.