PMID- 8832908
OWN - NLM
STAT- MEDLINE
DCOM- 19970522
LR  - 20031114
IS  - 1078-0297 (Print)
IS  - 1078-0297 (Linking)
VI  - 91
IP  - 2
DP  - 1996 Feb
TI  - Cytokine production-suppressive effect by macrophage colony-stimulating factor 
      (M-CSF).
PG  - 161-72
AB  - Macrophage colony-stimulating factor (M-CSF) is a protein which is necessary for 
      proliferation and differentiation of monocyte-macrophage precursor cells. We 
      examined the effect of M-CSF on the cytokine production using BCG sensitized mice 
      in vivo. On Day 0, BCG 1 mg/mouse was injected via the tail vein. Starting from 
      Day 2, M-CSF 30 mu g/mouse (1 X 10(8) U/mg) was injected every 2 days for a total 
      of six times (Day 2, 4, 6, 8, 10 and 12). On Day 14. LPS 25 mu g/mouse was 
      injected via the tail vein, and Interferon (IFN)/Tumor necrosis factor-alpha 
      (TNF-alpha) in serum were determined. The productions of IFN and TNF-alpha were 
      suppressed significantly. These cytokine production-suppressive effects of M-CSF 
      were found also in the in vitro experimental system using spleen cells collected. 
      On Day 14, spleen cells were collected and adjusted to 5 X 10(6) cells/ml. 20 
      micro-grams of LPS was added to 2ml of spleen cells and they were incubated in a 
      C02 incubator for 24 hours. IFN and TNF-alpha in the supernatant were determined. 
      In the experiment using nude mice, the cytokine suppressive effect of M-CSF was 
      not observed. MLR test was performed with spleen cells of C57BL/6 treated with 
      M-CSF as the responder cells, and spleen cells of C3H were treated with mitomycin 
      C as the stimulator cells. MLR was suppressed significantly by administration of 
      M-CSF. These results might possibly reflect the actual effect of M-CSF in the 
      living body, and the T-cell and cellular immunity might be concerned with the 
      mechanism of the cytokine production-suppressive effect of M-CSF.
FAU - Kojima, K
AU  - Kojima K
AD  - First Department of Surgery, Okayama University Medical School, Japan.
FAU - Naomoto, Y
AU  - Naomoto Y
FAU - Hizuta, A
AU  - Hizuta A
FAU - Tanaka, N
AU  - Tanaka N
FAU - Orita, K
AU  - Orita K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Res Commun Mol Pathol Pharmacol
JT  - Research communications in molecular pathology and pharmacology
JID - 9437512
RN  - 0 (BCG Vaccine)
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - BCG Vaccine/immunology
MH  - Cytokines/*biosynthesis
MH  - Depression, Chemical
MH  - Immunity, Cellular/drug effects/immunology
MH  - Lipopolysaccharides/pharmacology
MH  - Macrophage Colony-Stimulating Factor/*pharmacology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Nude
MH  - Spleen/cytology/drug effects/metabolism
EDAT- 1996/02/01 00:00
MHDA- 1996/02/01 00:01
CRDT- 1996/02/01 00:00
PHST- 1996/02/01 00:00 [pubmed]
PHST- 1996/02/01 00:01 [medline]
PHST- 1996/02/01 00:00 [entrez]
PST - ppublish
SO  - Res Commun Mol Pathol Pharmacol. 1996 Feb;91(2):161-72.