PMID- 8835352
OWN - NLM
STAT- MEDLINE
DCOM- 19961205
LR  - 20041117
IS  - 0146-6615 (Print)
IS  - 0146-6615 (Linking)
VI  - 48
IP  - 2
DP  - 1996 Feb
TI  - Search for human herpesvirus 6 and human cytomegalovirus in bronchoalveolar 
      lavage from patients with human immunodeficiency virus-1 and respiratory 
      disorders.
PG  - 179-83
AB  - Virus isolation and viral DNA detection by the polymerase chain reaction were 
      used to investigate the presence of human herpesvirus 6 (HHV-6) and human 
      cytomegalovirus (HCMV) in bronchoalveolar lavage from 34 human immunodeficiency 
      virus-1 (HIV-1)-infected patients with respiratory disorders. The aim was to 
      assess the presence of reactivated HHV-6 in lung tissues for a subsequent 
      evaluation of the frequency of virus involvement in respiratory clinical 
      manifestations in the course of HIV-1 infection. Bronchoalveolar lavage samples 
      were tested for the presence of HCMV, as a routine investigation within a 
      protocol monitoring opportunistic infections in symptomatic HIV-1 patients. 
      Whereas HCMV DNA was detected by the polymerase chain reaction in 12 
      bronchoalveolar lavage specimens, 10 of which were also positive for virus 
      isolation, all samples were negative for HHV-6 by both virological procedures. 
      The HHV-6 DNA finding in bronchoalveolar lavage from an HIV-1-seronegative 
      patient with renal carcinoma, investigated accidentally together with the 
      bronchoalveolar lavage specimens from HIV-1 seropositive patients, stressed the 
      HHV-6 polymerase chain reaction-negative results in the bronchoalveolar lavage 
      samples under study. It is concluded that the lung may be a target organ for HCMV 
      infection in HIV-1-seropositive patients affected by respiratory symptoms but 
      that this does not seem to be the case for HHV-6.
FAU - Portolani, M
AU  - Portolani M
AD  - Department of Biomedical Sciences, University of Modena, Italy.
FAU - Fabio, G
AU  - Fabio G
FAU - Pecorari, M
AU  - Pecorari M
FAU - Pietrosemoli, P
AU  - Pietrosemoli P
FAU - Meacci, M
AU  - Meacci M
FAU - Sabbatini, A M
AU  - Sabbatini AM
FAU - Cermelli, C
AU  - Cermelli C
FAU - De Rienzo, B
AU  - De Rienzo B
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Med Virol
JT  - Journal of medical virology
JID - 7705876
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Bronchoalveolar Lavage Fluid/*virology
MH  - Cohort Studies
MH  - Cytomegalovirus/*genetics/immunology
MH  - Cytomegalovirus Infections/complications/immunology/*virology
MH  - *DNA, Viral
MH  - HIV Seropositivity/*complications/immunology
MH  - HIV-1/isolation & purification
MH  - Herpesviridae Infections/complications/immunology/*virology
MH  - Herpesvirus 6, Human/*genetics/immunology
MH  - Humans
EDAT- 1996/02/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1996/02/01 00:00
PHST- 1996/02/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1996/02/01 00:00 [entrez]
AID - 10.1002/(SICI)1096-9071(199602)48:2<179::AID-JMV10>3.0.CO;2-A [pii]
AID - 10.1002/(SICI)1096-9071(199602)48:2<179::AID-JMV10>3.0.CO;2-A [doi]
PST - ppublish
SO  - J Med Virol. 1996 Feb;48(2):179-83. doi: 
      10.1002/(SICI)1096-9071(199602)48:2<179::AID-JMV10>3.0.CO;2-A.