PMID- 8835778
OWN - NLM
STAT- MEDLINE
DCOM- 19961206
LR  - 20200304
IS  - 0895-8696 (Print)
IS  - 0895-8696 (Linking)
VI  - 7
IP  - 1
DP  - 1996 Spring
TI  - The Ca2+/calmodulin-dependent protein kinase II-associated protein complex 
      isolated from chicken retina.
PG  - 1-12
AB  - Retinal cytosolic Ca2+/calmodulin-dependent protein kinase II (CaM KII) was 
      isolated from hatched 6-wk chicken retinae by ultracentrifugation and affinity 
      chromatography using calmodulin (CaM) and anti-CaM KII-alpha columns. Samples 
      from different fractions were examined with SDS-polyacrylamide gel 
      electrophoresis (SDS-PAGE) and silver staining or immunoblotting. Comparisons 
      were made between the final antibody affinity eluates from retina and forebrain. 
      Silver-stained gels showed that multiple proteins were present in the antibody 
      affinity eluates from retina, including major proteins of 178, 56, and 45 kDa and 
      several minor proteins. Immunoblots revealed that CaM KII-alpha was present in 
      eluates from the retina and forebrain. CaM KII-beta was present in the antibody 
      eluate from forebrain but not retina. The latter subunit was present in the crude 
      homogenates of the retina. Regarding the antibody eluate from retina, the 
      possibility that the major 56 kDa protein was tubulin was ruled out, but protein 
      tau (tau) and synapsin I were present. The presence of multiple proteins in the 
      antibody affinity eluate indicates that these proteins were coisolated in a CaM 
      KII-alpha-associated protein complex. The finding that protein tau and synapsin I 
      are associated with retinal CaM KII provides further insight into the mechanisms 
      underlying the function of the kinase in this tissue. The lack of cytosolic CaM 
      KII-beta subunit in the antibody affinity eluate from retina is indicative of a 
      brain region-specificity in subunit composition of the kinase.
FAU - Liu, N
AU  - Liu N
AD  - Department of Anatomical Sciences and Neurobiology, University of Louisville 
      School of Medicine, KY 40292, USA.
FAU - Cooper, N G
AU  - Cooper NG
LA  - eng
GR  - EY02708/EY/NEI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Mol Neurosci
JT  - Journal of molecular neuroscience : MN
JID - 9002991
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Calmodulin)
RN  - 0 (Synapsins)
RN  - 0 (tau Proteins)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Blotting, Western
MH  - Calcium-Calmodulin-Dependent Protein Kinase Type 2
MH  - Calcium-Calmodulin-Dependent Protein Kinases/chemistry/isolation & 
      purification/*metabolism
MH  - Calmodulin/immunology
MH  - Chickens
MH  - Chromatography, Affinity
MH  - Prosencephalon/chemistry/enzymology
MH  - Retina/chemistry/*enzymology
MH  - Substrate Specificity
MH  - Synapsins/analysis/metabolism
MH  - tau Proteins/analysis/metabolism
EDAT- 1996/01/01 00:00
MHDA- 2000/03/23 09:00
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 2000/03/23 09:00 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 10.1007/BF02736844 [doi]
PST - ppublish
SO  - J Mol Neurosci. 1996 Spring;7(1):1-12. doi: 10.1007/BF02736844.