PMID- 8837032
OWN - NLM
STAT- MEDLINE
DCOM- 19961211
LR  - 20191101
IS  - 1073-6085 (Print)
IS  - 1073-6085 (Linking)
VI  - 5
IP  - 3
DP  - 1996 Jun
TI  - Bead transfection of adherent cells. Gene transfer into adherent mammalian cells 
      using glass beads.
PG  - 259-61
AB  - Bead transfection is a simple, rapid, efficient, and cost-effective method of 
      gene transfer into adherent mammalian cells. It involves a brief incubation of 
      the cells with glass beads in a solution containing the DNA to be transferred. We 
      have optimized this technique using COS-7 (an SV40 transformed monkey kidney cell 
      line) and a transient expression assay for chloramphenicol acetyl transferase 
      (CAT). Stable transfection efficiency assessed using the selectable marker gene 
      neomycin phosphotransferase (NEOR) was 27% in COS-7 cells. As this technique 
      delivers high transfection efficiency with little manipulation of the exogenous 
      DNA and does not require the use of any viral sequences, it may be a useful 
      alternative method of gene delivery in the development of gene therapy protocols.
FAU - Matthews, K E
AU  - Matthews KE
AD  - Oncology Research Program, Toronto Hospital Research Institute, Canada.
FAU - Keating, A
AU  - Keating A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Mol Biotechnol
JT  - Molecular biotechnology
JID - 9423533
RN  - 0 (DNA, Recombinant)
RN  - EC 2.3.1.28 (Chloramphenicol O-Acetyltransferase)
RN  - I16QD7X297 (Neomycin)
SB  - IM
MH  - Animals
MH  - COS Cells
MH  - Cell Adhesion
MH  - Chloramphenicol O-Acetyltransferase/genetics
MH  - DNA, Recombinant
MH  - *Gene Transfer Techniques
MH  - Glass
MH  - Neomycin
MH  - Transfection/*methods
EDAT- 1996/06/01 00:00
MHDA- 1996/06/01 00:01
CRDT- 1996/06/01 00:00
PHST- 1996/06/01 00:00 [pubmed]
PHST- 1996/06/01 00:01 [medline]
PHST- 1996/06/01 00:00 [entrez]
AID - 10.1007/BF02900364 [doi]
PST - ppublish
SO  - Mol Biotechnol. 1996 Jun;5(3):259-61. doi: 10.1007/BF02900364.