PMID- 8837058
OWN - NLM
STAT- MEDLINE
DCOM- 19961220
LR  - 20190920
IS  - 0161-6412 (Print)
IS  - 0161-6412 (Linking)
VI  - 18
IP  - 3
DP  - 1996 Jun
TI  - Preliminary morphometric study of tumor necrosis factor-alpha (TNF alpha)-induced 
      rabbit optic neuropathy.
PG  - 233-6
AB  - Intravitreal injections of tumor necrosis factor-alpha (TNF alpha) induce 
      morphological changes in the optic nerve suggesting a primary TNF alpha-induced 
      optic neuropathy. A preliminary morphometric analysis of axonal loss and 
      degeneration in optic nerves of a TNF alpha rabbit eye model was conducted. 
      Fifteen rabbits were injected in one eye only with TNF alpha (final doses: 2 U, 
      20 U and 200 U respectively), and optic nerve cross-sections were examined using 
      a semi-automated image analysis system, at varying time intervals for up to 24 
      weeks post-injection. Control optic nerves (uninjected or injected with diluent 
      only) were also assessed. Intact, degenerating and demyelinated axons were 
      visible in TNF alpha-exposed optic nerves. Considerable variation was observed 
      between TNF alpha-exposed optic nerves, however the intact axon density 
      consistently decreased in the 200 U TNF alpha group, although not in the 2 U and 
      20 U groups. Overall, TNF alpha-exposed optic nerves had significantly more 
      degenerating profiles compared to control optic nerves. These observations 
      suggest a dose-dependent axonal loss and degeneration associated with the 
      morphological changes induced in rabbit optic nerves following intravitreal 
      injection of TNF alpha.
FAU - Madigan, M C
AU  - Madigan MC
AD  - Department of Clinical Ophthalmology, University of Sydney, N.S.W., Australia.
FAU - Rao, N S
AU  - Rao NS
FAU - Tenhula, W N
AU  - Tenhula WN
FAU - Sadun, A A
AU  - Sadun AA
LA  - eng
GR  - R01-EY-08145/EY/NEI NIH HHS/United States
GR  - R01-EY-09510/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Neurol Res
JT  - Neurological research
JID - 7905298
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Optic Neuritis/chemically induced/*pathology
MH  - Rabbits
MH  - Time Factors
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1996/06/01 00:00
MHDA- 1996/06/01 00:01
CRDT- 1996/06/01 00:00
PHST- 1996/06/01 00:00 [pubmed]
PHST- 1996/06/01 00:01 [medline]
PHST- 1996/06/01 00:00 [entrez]
AID - 10.1080/01616412.1996.11740410 [doi]
PST - ppublish
SO  - Neurol Res. 1996 Jun;18(3):233-6. doi: 10.1080/01616412.1996.11740410.