PMID- 8839870
OWN - NLM
STAT- MEDLINE
DCOM- 19961107
LR  - 20231213
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 88
IP  - 7
DP  - 1996 Oct 1
TI  - Modulation of erythrocyte potassium chloride cotransport, potassium content, and 
      density by dietary magnesium intake in transgenic SAD mouse.
PG  - 2738-44
AB  - Prevention of erythrocyte dehydration is a potential therapeutic strategy for 
      sickle cell disease. Increasing erythrocyte magnesium (Mg) could inhibit sickle 
      cell dehydration by increasing chloride (CI) and water content and by inhibiting 
      potassium chloride (K-CI) cotransport. In transgenic SAD 1 and (control) C57BL/6 
      normal mice, we investigated the effect of 2 weeks of diet with either low Mg (6 
      +/- 2 mg/kg body weight/d) or high Mg (1,000 +/- 20 mg/kg body weight/ d), in 
      comparison with a diet of standard Mg (400 +/- 20 mg/ kg body weight/d). The 
      high-Mg diet increased SAD 1 erythrocyte Mg and K contents and reduced K-CI 
      cotransport activity, mean corpuscular hemoglobin concentration (MCHC), cell 
      density, and reticulocyte count. SAD 1 mice treated with low-Mg diet showed a 
      significant reduction in erythrocyte Mg and K contents and increases in K-CI 
      cotransport, MCHC, cell density, and reticulocyte counts. In SAD 1 mice, 
      hematocrit (Hct) and hemoglobin (Hb) decreased significantly with low Mg diet and 
      increased significantly with high-Mg diet. The C57BL/6 controls showed 
      significant changes only in erythrocyte Mg and K content, and K-CI cotransport 
      activities, similar to those observed in SAD 1 mice. Thus, in the SAD 1 mouse, 
      changes in dietary Mg modulate K-CI cotransport, modify erythrocyte dehydration, 
      and ultimately affect Hb levels.
FAU - De Franceschi, L
AU  - De Franceschi L
AD  - Department of Internal Medicine, University of Verona, Italy.
FAU - Beuzard, Y
AU  - Beuzard Y
FAU - Jouault, H
AU  - Jouault H
FAU - Brugnara, C
AU  - Brugnara C
LA  - eng
GR  - P60-HL15157/HL/NHLBI NIH HHS/United States
GR  - R01-DK50422/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Carrier Proteins)
RN  - 0 (Chlorides)
RN  - 0 (Hemoglobin, Sickle)
RN  - 0 (Hemoglobins)
RN  - 0 (Symporters)
RN  - I38ZP9992A (Magnesium)
RN  - RWP5GA015D (Potassium)
SB  - IM
MH  - Anemia, Sickle Cell/blood/complications/*drug therapy/genetics
MH  - Animals
MH  - Biological Transport/drug effects
MH  - Carrier Proteins/*blood
MH  - Chlorides/*blood
MH  - Diet
MH  - Disease Models, Animal
MH  - Erythrocytes, Abnormal/chemistry/*drug effects
MH  - Female
MH  - Hematocrit
MH  - Hemoglobin, Sickle/chemistry/genetics
MH  - Hemoglobins/analysis
MH  - Magnesium/administration & dosage/blood/*therapeutic use
MH  - Magnesium Deficiency/blood/complications
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Osmotic Fragility/drug effects
MH  - Potassium/*blood
MH  - Reticulocyte Count/drug effects
MH  - *Symporters
MH  - K Cl- Cotransporters
EDAT- 1996/10/01 00:00
MHDA- 1996/10/01 00:01
CRDT- 1996/10/01 00:00
PHST- 1996/10/01 00:00 [pubmed]
PHST- 1996/10/01 00:01 [medline]
PHST- 1996/10/01 00:00 [entrez]
AID - S0006-4971(20)62372-2 [pii]
PST - ppublish
SO  - Blood. 1996 Oct 1;88(7):2738-44.