PMID- 8840467
OWN - NLM
STAT- MEDLINE
DCOM- 19961223
LR  - 20190727
IS  - 0049-3848 (Print)
IS  - 0049-3848 (Linking)
VI  - 83
IP  - 3
DP  - 1996 Aug 1
TI  - Interaction of a new depolymerized holothurian glycosaminoglycan with proteins in 
      human plasma.
PG  - 253-64
AB  - In a rat template bleeding model, depolymerized holothurian glycosaminoglycan 
      (DHG) prolonged bleeding time at 30 mg/kg i.v. but unfractionated heparin (UFH) 
      had the same effect at 1 mg/kg i.v., indicating that DHG is much less bleeding 
      than UFH. To characterize this difference, we examined the affinity of DHG for 
      plasma proteins by means of a glycosaminoglycan-conjugated cellulofine column in 
      comparison with that of UFH. The DHG column strongly bound factor V, factor IX, 
      protein S, histidine-rich glycoprotein, platelet factor 4 (PF4), 
      beta-thromboglobulin, von Willebrand factor, fibronectin, and heparin cofactor 
      II, but did not bind fibrinogen, prothrombin, factor VII, protein C, antithrombin 
      III (ATIII), plasminogen or alpha 2-plasmin inhibitor. The profile of protein 
      binding to the UFH column was almost the same as that of the DHG column except 
      that ATIII showed affinity for UFH. One of the reasons why DHG caused much less 
      bleeding than UFH is thus suggested to be the differences in their affinity for 
      ATIII in plasma.
FAU - Minamiguchi, K
AU  - Minamiguchi K
AD  - Pharmacology Research Laboratory, Taiho Pharmaceutical Co., Ltd., Tokushima, 
      Japan.
FAU - Nagase, H
AU  - Nagase H
FAU - Kitazato, K T
AU  - Kitazato KT
FAU - Kitazato, K
AU  - Kitazato K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Thromb Res
JT  - Thrombosis research
JID - 0326377
RN  - 0 (Blood Proteins)
RN  - 0 (Glycosaminoglycans)
SB  - IM
MH  - Animals
MH  - Bleeding Time
MH  - Blood Proteins/*metabolism
MH  - Glycosaminoglycans/*metabolism/pharmacology
MH  - Hemorrhage/*metabolism
MH  - Humans
MH  - Protein Binding
MH  - Rats
EDAT- 1996/08/01 00:00
MHDA- 1996/08/01 00:01
CRDT- 1996/08/01 00:00
PHST- 1996/08/01 00:00 [pubmed]
PHST- 1996/08/01 00:01 [medline]
PHST- 1996/08/01 00:00 [entrez]
AID - 0049-3848(96)00134-X [pii]
AID - 10.1016/0049-3848(96)00134-x [doi]
PST - ppublish
SO  - Thromb Res. 1996 Aug 1;83(3):253-64. doi: 10.1016/0049-3848(96)00134-x.