PMID- 8840522
OWN - NLM
STAT- MEDLINE
DCOM- 19961210
LR  - 20191101
IS  - 0962-8819 (Print)
IS  - 0962-8819 (Linking)
VI  - 5
IP  - 6
DP  - 1996 Nov
TI  - Rapid fluorescence in situ hybridization on interphasic nuclei to discriminate 
      between homozygous and heterozygous transgenic mice.
PG  - 397-403
AB  - Homozygous and heterozygous transgenic mice of the Tg152 line overexpressing the 
      human copper/zinc superoxide dismutase (hSOD-1) were rapidly differentiated by 
      fluorescence in situ hybridization (FISH) using interphase lymphocyte nuclei. We 
      have devised a simple and fast method for preparing interphase nuclei with very 
      small quantities of whole mouse blood, avoiding several steps of the classical 
      FISH technique. Lymphocyte separation and cell culture were not required. This 
      technique provides an excellent tool for the unambiguous detection of homozygous 
      and heterozygous transgenic mice in a litter. It can be used to check young 
      animals since 2 microliters of whole blood is sufficient. We also show that in 
      this transgenic line numerous copies of the hSOD-1 transgene are integrated at a 
      single autosomal locus, in tandem head-to-tail organization.
FAU - Paris, D
AU  - Paris D
AD  - CNRS URA 1335, Faculte de Medecine Necker-Enfants Malades, Paris, France.
FAU - Toyama, K
AU  - Toyama K
FAU - Megarbane, A
AU  - Megarbane A
FAU - Casanova, P M
AU  - Casanova PM
FAU - Sinet, P M
AU  - Sinet PM
FAU - London, J
AU  - London J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Transgenic Res
JT  - Transgenic research
JID - 9209120
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Animals
MH  - Blotting, Southern
MH  - Breeding
MH  - Cell Nucleus/*genetics
MH  - Chromosomes
MH  - Female
MH  - Gene Frequency
MH  - Heterozygote
MH  - *Homozygote
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lymphocytes/chemistry/enzymology
MH  - Metaphase
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Mice, Transgenic
MH  - Polymerase Chain Reaction
MH  - Superoxide Dismutase/genetics/metabolism
EDAT- 1996/11/01 00:00
MHDA- 1996/11/01 00:01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 1996/11/01 00:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
AID - 10.1007/BF01980204 [doi]
PST - ppublish
SO  - Transgenic Res. 1996 Nov;5(6):397-403. doi: 10.1007/BF01980204.