PMID- 8841641
OWN - NLM
STAT- MEDLINE
DCOM- 19961218
LR  - 20131121
IS  - 0261-3166 (Print)
IS  - 0261-3166 (Linking)
IP  - 34
DP  - 1995
TI  - DNA diagnosis of malaria using microtiter plate-hybridization.
PG  - 241-2
AB  - We have developed a new diagnostic method "microtiter plate-hybridization" (MPH) 
      for the detection of human malaria parasite in which the target DNA sequence of 
      the 18S ribosomal RNA gene is amplified by polymerase chain reaction and 
      hybridized with the species-specific probes immobilized on a microtiter well. The 
      PCR products bound on a well are visualized by the biotin-streptavidin system and 
      the following chromogenic reaction. This method has allowed us to detect and 
      identify the four species of human malaria parasites. We obtained blood samples 
      by finger puncture from 435 donors in Japan, Solomon Island and Vietnam. The 
      results of our method showed good correlation with the results of Giemsa staining 
      microscopy. Furthermore, we developed a new system for the detection of new human 
      malaria parasites. This system involves an acridine orange (AO) staining 
      microscopic examination and "microtiter plate-hybridization". Using the system, 
      we found a case of new variant of Plasmodium ovale whose PCR-amplified DNA did 
      not hybridize with a probe for typical P. ovale in Vietnam. These results 
      indicate that our new method can serve as a useful tool for the clinical 
      management, epidemiological research of malaria, and investigation of the new 
      type of malaria parasite.
FAU - Miyake, H
AU  - Miyake H
AD  - Faculty of Pharmaceutical Sciences, Okayama University, Japan.
FAU - Kim, H S
AU  - Kim HS
FAU - Kawai, S
AU  - Kawai S
FAU - Yamane, A
AU  - Yamane A
FAU - Kimura, M
AU  - Kimura M
FAU - Wataya, Y
AU  - Wataya Y
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nucleic Acids Symp Ser
JT  - Nucleic acids symposium series
JID - 8007206
RN  - 0 (DNA, Protozoan)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (RNA, Protozoan)
RN  - 0 (RNA, Ribosomal, 18S)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - DNA, Protozoan/*blood/*genetics
MH  - Evaluation Studies as Topic
MH  - Fluorescent Dyes
MH  - Genes, Protozoan
MH  - Genetic Variation
MH  - Humans
MH  - Malaria/*diagnosis/epidemiology/parasitology
MH  - Melanesia/epidemiology
MH  - Molecular Epidemiology
MH  - Nucleic Acid Hybridization
MH  - Plasmodium/classification/*genetics/isolation & purification
MH  - Polymerase Chain Reaction
MH  - RNA, Protozoan/genetics
MH  - RNA, Ribosomal, 18S/genetics
MH  - Species Specificity
MH  - Vietnam/epidemiology
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
PST - ppublish
SO  - Nucleic Acids Symp Ser. 1995;(34):241-2.