PMID- 8842155
OWN - NLM
STAT- MEDLINE
DCOM- 19961125
LR  - 20190725
IS  - 0026-8925 (Print)
IS  - 0026-8925 (Linking)
VI  - 252
IP  - 3
DP  - 1996 Sep 13
TI  - Cloning and molecular analysis of cDNAs encoding three sucrose phosphate synthase 
      isoforms from a citrus fruit (Citrus unshiu Marc.).
PG  - 346-51
AB  - Three partial cDNA clones (pSPS1, pSPS2 and pSPS3) encoding sucrose phosphate 
      synthases (SPS) were isolated by Reverse Transcription (RT)-PCR using 
      first-strand cDNA prepared from the leaf or fruit of citrus (Citrus unshiu 
      Marc.). The nucleotide and deduced amino acid sequences of the three clones 
      showed significant similarities to SPS previously isolated in other plants. A 
      full-length, cDNA clone, CitSPS1, was isolated from a fruit (juice sacs and pulp 
      segment) cDNA library using one (pSPS1) of the three partial clones as a probe. 
      The 3539-bp CitSPS1 clone coded for a 1057-amino acid polypeptide with a 
      predicted molecular mass of 117.8 kDa. The amino acid sequence deduced from the 
      CitSPS1 clone showed homology with SPS from maize (55.8% identity) and spinach 
      (74.0% identity). Genomic Southern blot analysis suggested that CitSPS1 clone 
      represents a lowcopy-number gene. RNA blot analysis of leaf, flower and fruit 
      showed that CitSPS1 and pSPS2 were expressed in all organs. However, the levels 
      of expression of CitSPS1 in young leaves, flowers and immature fruits were low, 
      but high in mature leaves, and fruit. pSPS2 transcripts were barely detectable in 
      young leaves and immature fruits, low in mature leaves, and high in flowers and 
      mature fruits. pSPS3 transcripts were only detected in young and in mature 
      leaves.
FAU - Komatsu, A
AU  - Komatsu A
AD  - Faculty of Agriculture, Meiji University, Kanagawa, Japan.
FAU - Takanokura, Y
AU  - Takanokura Y
FAU - Omura, M
AU  - Omura M
FAU - Akihama, T
AU  - Akihama T
LA  - eng
SI  - GENBANK/AB006319
SI  - GENBANK/AB006660
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Mol Gen Genet
JT  - Molecular & general genetics : MGG
JID - 0125036
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA, Plant)
RN  - 0 (Isoenzymes)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Plant)
RN  - EC 2.4.1.- (Glucosyltransferases)
RN  - EC 2.4.1.14 (sucrose-phosphate synthase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Citrus/*enzymology
MH  - Cloning, Molecular
MH  - DNA Probes
MH  - DNA, Complementary
MH  - DNA, Plant
MH  - Gene Expression
MH  - Glucosyltransferases/*genetics/isolation & purification
MH  - Isoenzymes/*genetics/isolation & purification
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger
MH  - RNA, Plant
MH  - Sequence Homology, Amino Acid
EDAT- 1996/09/13 00:00
MHDA- 1996/09/13 00:01
CRDT- 1996/09/13 00:00
PHST- 1996/09/13 00:00 [pubmed]
PHST- 1996/09/13 00:01 [medline]
PHST- 1996/09/13 00:00 [entrez]
AID - 10.1007/BF02173781 [doi]
PST - ppublish
SO  - Mol Gen Genet. 1996 Sep 13;252(3):346-51. doi: 10.1007/BF02173781.