PMID- 8844126
OWN - NLM
STAT- MEDLINE
DCOM- 19970102
LR  - 20190905
IS  - 0340-3696 (Print)
IS  - 0340-3696 (Linking)
VI  - 288
IP  - 8
DP  - 1996 Jul
TI  - Effects of basophil-priming and stimulating cytokines on histamine release from 
      isolated human skin mast cells.
PG  - 463-8
AB  - Cell priming and stimulation of different cytokines (which include chemokines and 
      growth factors) are typical features of human basophils. Recently, it has been 
      shown that the macrophage chemotactic protein-1 (MCP-1), RANTES and macrophage 
      inflammatory protein-1 alpha (MIP-1 alpha) are potent direct secretagogues for 
      human basophils and that interleukin-3 (IL-3), IL-5 and granulocyte/macrophage 
      colony-stimulating factor (GM-CSF) are priming factors for subsequent 
      potentiation of mediator release from basophils induced by different stimuli. 
      This observation may be clinically important for the activation and recruitment 
      of inflammatory cells in different immune responses of the skin (e.g. late-phase 
      reactions). The aim of the present study was to investigate whether cytokines and 
      chemokines are also capable of priming or stimulating isolated human skin mast 
      cells (SMC). SMC were either stimulated directly with the cytokines alone or 
      preincubated with these factors for 10 min before being activated with suboptimal 
      concentrations of anti-IgE, A23187 or substance P. IL-3, IL-5, GM-CSF, platelet 
      factor-4 (PF-4), IL-8, MCP-1 and MIP-1 alpha (each at concentrations of 1 ng/ml 
      to 1 microgram/ml, log steps) did not significantly modulate histamine release 
      from SMC induced by the three different secretagogues. RANTES exhibited a weak 
      but significant potentiating effect on IgE-mediated activation. Stem cell factor 
      (SCF) as a positive control was able to prime mast cell histamine release 
      strongly. In addition, PF-4, MCP-1, RANTES and MIP-1 alpha were incapable of 
      inducing direct histamine release from SMC. In experiments with isolated human 
      peripheral basophils, however, we observed potent Fc epsilon RI-mediated priming 
      effects evoked through IL-3, IL-5 and GM-CSF. We conclude that SMC derived from 
      healthy donors are not targets of (immuno)modulatory factors that prime or 
      stimulate basophils.
FAU - Nitschke, M
AU  - Nitschke M
AD  - Department of Dermatology, Medical University of Lubeck, Germany.
FAU - Sohn, K
AU  - Sohn K
FAU - Dieckmann, D
AU  - Dieckmann D
FAU - Gibbs, B F
AU  - Gibbs BF
FAU - Wolff, H H
AU  - Wolff HH
FAU - Amon, U
AU  - Amon U
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Arch Dermatol Res
JT  - Archives of dermatological research
JID - 8000462
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-3)
RN  - 0 (Interleukin-5)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Basophils/*physiology
MH  - Chemokine CCL2/pharmacology
MH  - Chemokine CCL4
MH  - Chemokine CCL5/pharmacology
MH  - Cytokines/*pharmacology
MH  - Evaluation Studies as Topic
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Histamine Release/*drug effects
MH  - Humans
MH  - In Vitro Techniques
MH  - Interleukin-3/pharmacology
MH  - Interleukin-5/pharmacology
MH  - Macrophage Inflammatory Proteins/pharmacology
MH  - Mast Cells/*drug effects/metabolism
MH  - Skin/cytology/*drug effects/metabolism
MH  - Stimulation, Chemical
EDAT- 1996/07/01 00:00
MHDA- 1996/07/01 00:01
CRDT- 1996/07/01 00:00
PHST- 1996/07/01 00:00 [pubmed]
PHST- 1996/07/01 00:01 [medline]
PHST- 1996/07/01 00:00 [entrez]
AID - 10.1007/BF02505236 [doi]
PST - ppublish
SO  - Arch Dermatol Res. 1996 Jul;288(8):463-8. doi: 10.1007/BF02505236.