PMID- 8844337
OWN - NLM
STAT- MEDLINE
DCOM- 19961224
LR  - 20211203
IS  - 0393-974X (Print)
IS  - 0393-974X (Linking)
VI  - 9
IP  - 4
DP  - 1995 Oct-Dec
TI  - Protein synthesis in TE 671/RD (human rabdomiosarcoma) cells treated with 
      thapsigargin and hyperthermia: impairment of HSP 70 induction.
PG  - 132-8
AB  - In this study we considered the quantitative and qualitative changes of protein 
      synthetic activity occurring in TE 671/RD cells treated with thapsigargin (TG), 
      with hyperthermia (HT) or with a combination of both these agents. In cells 
      treated with TG (100 nM, continuous exposure), the overall protein synthetic 
      activity was initially inhibited but subsequently recovered to about 60% of the 
      initial level. Chronic TG exposure was also able to induce the expression of GRP 
      78. The rate of synthesis of GRP 78, after a lag period of about 2 h, increased 
      gradually to reach a maximum (9-fold induction) after 6 h of TG-treatment and was 
      then maintained at that level up to 18 h. A weak induction of GRP 94 was observed 
      following 6-8 h of continuous exposure to TG. In cells treated with HT (43 
      degrees C for 30 min), a typical heat shock response was observed: in particular, 
      the relative rate of synthesis of HSP 70 (the major heat-inducible mammalian heat 
      shock protein) was increased 10-fold over the constitutive level. The 
      heat-promoted induction of HSP 70 was significantly reduced by concomitant or 
      previous exposure to TG. When TG and HT were administred simultaneously, the 
      increase in HSP 70 synthesis was only 4.7-fold over the control level, while in 
      cells pre-treated for 1 h with TG before the hyperthermic challenge the rate of 
      HSP 70 synthesis was only stimulated 2-fold. In both these conditions, by 
      contrast, it was apparent that HT did not affect the TG-promoted induction of GRP 
      78. The correlations between the TG-induced mobilization of cytosolic Ca2+ and 
      the effects on protein synthesis are discussed.
FAU - Delpino, A
AU  - Delpino A
AD  - Laboratory of Biophysics, Regina Elena Institute for Cancer Research, Roma, 
      Italy.
FAU - Piselli, P
AU  - Piselli P
FAU - Mangano, G
AU  - Mangano G
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - J Biol Regul Homeost Agents
JT  - Journal of biological regulators and homeostatic agents
JID - 8809253
RN  - 0 (Carrier Proteins)
RN  - 0 (Endoplasmic Reticulum Chaperone BiP)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Molecular Chaperones)
RN  - 0 (Protein Synthesis Inhibitors)
RN  - 0 (Proteins)
RN  - 67526-95-8 (Thapsigargin)
SB  - IM
MH  - Carrier Proteins/biosynthesis/drug effects
MH  - Endoplasmic Reticulum Chaperone BiP
MH  - Enzyme Inhibitors/pharmacology
MH  - HSP70 Heat-Shock Proteins/biosynthesis/drug effects
MH  - *Heat-Shock Proteins
MH  - Heat-Shock Response/*physiology
MH  - Humans
MH  - Molecular Chaperones/biosynthesis/drug effects
MH  - *Protein Biosynthesis
MH  - Protein Synthesis Inhibitors/pharmacology
MH  - Proteins/drug effects
MH  - Rhabdomyosarcoma/*drug therapy/*metabolism/pathology
MH  - Temperature
MH  - Thapsigargin/*pharmacology
EDAT- 1995/10/01 00:00
MHDA- 1995/10/01 00:01
CRDT- 1995/10/01 00:00
PHST- 1995/10/01 00:00 [pubmed]
PHST- 1995/10/01 00:01 [medline]
PHST- 1995/10/01 00:00 [entrez]
PST - ppublish
SO  - J Biol Regul Homeost Agents. 1995 Oct-Dec;9(4):132-8.