PMID- 8844470
OWN - NLM
STAT- MEDLINE
DCOM- 19961223
LR  - 20190920
IS  - 0944-1174 (Print)
IS  - 0944-1174 (Linking)
VI  - 31
IP  - 4
DP  - 1996 Aug
TI  - Chromosome spreading techniques for primary gastrointestinal tumors.
PG  - 505-11
AB  - The requirement for well spread out chromosomes for the cytogenetic analysis of 
      primary gastrointestinal tumors led us to develop new techniques. These 
      techniques involved two main procedures: (1) preliminary incubation with culture 
      medium in the presence of collagenase, Dispase, and colcemid, for 3 h, and (2) 
      treatment with an extremely hypotonic solution (0.044M KCl) for 30 min. The 
      techniques were applied to 11 gastrointestinal malignancies (including 1 early 
      gastric cancer and 1 metastatic liver lesion of colon cancer) and significant 
      increases (P < 0.01) in the number of metaphases of analyzable karyotypes were 
      obtained, compared with a previous method in which the standard hypotonic 
      molarity of KCL (0.075 M) was employed. The mean value for metaphase numbers of 
      the analyzable karyotypes was 37.0 +/- 3.7% in the 5 gastric cancers and 44.7 +/- 
      4.8% in the 5 colon cancers and 1 metastatic lesion. These values were three 
      times and more than twice, respectively, the values obtained by the previous 
      method. A fluorescence in situ hybridization (FISH) study was carried out on one 
      cologenic tumor, the alpha-satellite centromere-specific probe 17 being used. 
      Deletion of the long arm of chromosome 17 was demonstrated. The method proposed 
      here could yield a sufficient number of metaphases without the use of tissue 
      culture that might cause alteration of karyotype. It can be employed with small 
      biopsy specimens and in studies utilizing the FISH technique.
FAU - Sasai, K
AU  - Sasai K
AD  - Department of Radiology, Yamaguchi University, School of Medicine, Japan.
FAU - Tanaka, R
AU  - Tanaka R
FAU - Kawamura, M
AU  - Kawamura M
FAU - Honjo, K
AU  - Honjo K
FAU - Matsunaga, N
AU  - Matsunaga N
FAU - Nakada, T
AU  - Nakada T
FAU - Homma, K
AU  - Homma K
FAU - Fujimura, H
AU  - Fujimura H
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - J Gastroenterol
JT  - Journal of gastroenterology
JID - 9430794
RN  - 0 (Culture Media)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Culture Media
MH  - Cytogenetics/methods
MH  - Female
MH  - Gastrointestinal Neoplasms/*genetics/pathology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping/methods
MH  - Male
MH  - Metaphase
MH  - Middle Aged
EDAT- 1996/08/01 00:00
MHDA- 1996/08/01 00:01
CRDT- 1996/08/01 00:00
PHST- 1996/08/01 00:00 [pubmed]
PHST- 1996/08/01 00:01 [medline]
PHST- 1996/08/01 00:00 [entrez]
AID - 10.1007/BF02355049 [doi]
PST - ppublish
SO  - J Gastroenterol. 1996 Aug;31(4):505-11. doi: 10.1007/BF02355049.