PMID- 8844594
OWN - NLM
STAT- MEDLINE
DCOM- 19961223
LR  - 20190516
IS  - 0916-7250 (Print)
IS  - 0916-7250 (Linking)
VI  - 58
IP  - 7
DP  - 1996 Jul
TI  - Heterogeneity in the origin and immunophenotypes of "histiocytic" cells in 
      transplantable rat malignant fibrous histiocytoma.
PG  - 603-9
AB  - To clarify the origin and nature of "histiocytic" cells in malignant fibrous 
      histiocytoma (MFH), immunoreactivities to rat macrophage/histiocyte-specific 
      monoclonal antibodies (ED1 and ED2) and monocyte chemoattractant protein-1 
      (MCP-1) production were investigated using rat transplantable MFH-derived cloned 
      cells (undifferentiated MT-8 and fibrohistiocytic MT-9). In different cultures, 
      the positive rates for ED1 and ED2 ranged from 2.5% to 26.0% in MT-8, and from 
      6.0% to 40.0% in MT-9. In homotransplants, ED1-positive cells were frequently 
      observed in MT-9 tumors, but barely seen in MT-8 tumors. In both MT-8 and MT-9 
      tumors, ED2-positive cells were not detected within tumors. Immunophenotypic 
      characteristics of MT-8 and MT-9 to these antibodies seemed to be easily altered 
      depending on in vitro and in vivo conditions. By ELISA, MCP-1 was hardly detected 
      in culture supernatants of MT-8 and MT-9, but its level was very high in sera of 
      MT-8- and MT-9-tumor-bearing rats. In rats with these tumors, the number of 
      circulating monocytes was significantly increased and the presence of factors 
      stimulating macrophage proliferation was demonstrated by the colony formation 
      assay using rat bone marrow cells. Histiocytic cells in MT-8 and MT-9 tumors that 
      were produced in nude mice reacted only with antibody to mouse 
      macrophage-associated antigen. This suggests the existence of non-neoplastic, 
      infiltrated macrophages of host origin that were induced probably by MCP-1 and 
      factors stimulating macrophage proliferation. The present studies indicate the 
      presence of heterogeneities in the origin and immunophenotypes of "histiocytic" 
      cells in rat MFH.
FAU - Yamate, J
AU  - Yamate J
AD  - Department of Veterinary Pathology, College of Agriculture, Osaka Prefecture 
      University, Japan.
FAU - Tajima, M
AU  - Tajima M
FAU - Shibuya, K
AU  - Shibuya K
FAU - Kuwamura, M
AU  - Kuwamura M
FAU - Kotani, T
AU  - Kotani T
FAU - Sakuma, S
AU  - Sakuma S
FAU - Takeya, M
AU  - Takeya M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - J Vet Med Sci
JT  - The Journal of veterinary medical science
JID - 9105360
RN  - 0 (Chemokine CCL2)
SB  - IM
MH  - Animals
MH  - Bone Marrow/pathology
MH  - Chemokine CCL2/analysis
MH  - Clone Cells
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Histiocytoma, Benign Fibrous/*immunology/pathology
MH  - Immunophenotyping
MH  - Kinetics
MH  - Liver/immunology/pathology
MH  - Macrophages/immunology/pathology
MH  - Mice
MH  - Mice, Nude
MH  - Rats
MH  - Spleen/immunology/pathology
MH  - Transplantation, Heterologous
MH  - Tumor Cells, Cultured
EDAT- 1996/07/01 00:00
MHDA- 1996/07/01 00:01
CRDT- 1996/07/01 00:00
PHST- 1996/07/01 00:00 [pubmed]
PHST- 1996/07/01 00:01 [medline]
PHST- 1996/07/01 00:00 [entrez]
AID - 10.1292/jvms.58.603 [doi]
PST - ppublish
SO  - J Vet Med Sci. 1996 Jul;58(7):603-9. doi: 10.1292/jvms.58.603.