PMID- 8845459
OWN - NLM
STAT- MEDLINE
DCOM- 19961024
LR  - 20190914
IS  - 0957-5235 (Print)
IS  - 0957-5235 (Linking)
VI  - 7
IP  - 1
DP  - 1996 Jan
TI  - Inhibition of thrombin generation by heparin and LMW heparins: a comparison of 
      chromogenic and clotting methods.
PG  - 24-30
AB  - Inhibition of thrombin generation by heparin and low-molecular-weight (LMW) 
      heparins is an important parameter which relates to their anticoagulant actions 
      in vivo. Previous studies in our laboratory used a clotting assay for assessment 
      of thrombin generation but other published studies have used a chromogenic 
      method. We have therefore measured the inhibition of thrombin generation by 
      unfractionated heparin (UFH) and LMW heparins by a modified chromogenic method 
      using microtitre plates and compared the results with the clotting method. The 
      degree of inhibition of thrombin generation when calculated from both peak 
      thrombin concentrations and areas under the curve in the chromogenic assay was 
      the same. When the activities of each heparin were expressed as EC80, i.e. 
      concentrations required for 80% inhibition of thrombin generation, the EC80 were 
      higher in the chromogenic assay than in the clotting system. However when the 
      potencies of the LMW heparins were expressed as percentages of that of the UFH 
      standard by parallel line analysis, the differences between clotting and 
      chromogenic assay results were small and not statistically significant (P > 
      0.05). This study demonstrates the feasibility of measuring inhibition of 
      thrombin generation by a modified chromogenic method using microtitre plates, and 
      shows that the results with this method are similar to those obtained with the 
      clotting method.
FAU - Houbouyan, L
AU  - Houbouyan L
AD  - Hopital Ambroise-Pare, Boulogne, France.
FAU - Padilla, A
AU  - Padilla A
FAU - Gray, E
AU  - Gray E
FAU - Longstaff, C
AU  - Longstaff C
FAU - Barrowcliffe, T W
AU  - Barrowcliffe TW
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Blood Coagul Fibrinolysis
JT  - Blood coagulation & fibrinolysis : an international journal in haemostasis and 
      thrombosis
JID - 9102551
RN  - 0 (Anticoagulants)
RN  - 0 (Chromogenic Compounds)
RN  - 0 (Heparin, Low-Molecular-Weight)
RN  - 9005-49-6 (Heparin)
RN  - EC 3.4.21.5 (Thrombin)
SB  - IM
MH  - Anticoagulants/*pharmacology
MH  - *Blood Coagulation Tests
MH  - *Chromogenic Compounds
MH  - Heparin/*pharmacology
MH  - Heparin, Low-Molecular-Weight/*pharmacology
MH  - Humans
MH  - Linear Models
MH  - Reference Standards
MH  - Spectrophotometry
MH  - Thrombin/*biosynthesis
MH  - Titrimetry/instrumentation
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 10.1097/00001721-199601000-00003 [doi]
PST - ppublish
SO  - Blood Coagul Fibrinolysis. 1996 Jan;7(1):24-30. doi: 
      10.1097/00001721-199601000-00003.