PMID- 8846121
OWN - NLM
STAT- MEDLINE
DCOM- 19961021
LR  - 20190914
IS  - 1077-4114 (Print)
IS  - 1077-4114 (Linking)
VI  - 18
IP  - 2
DP  - 1996 May
TI  - Comparison of fluorescence in situ hybridization, cytogenetic analysis, and DNA 
      index analysis to detect chromosomes 4 and 10 aneuploidy in pediatric acute 
      lymphoblastic leukemia: a Pediatric Oncology Group study.
PG  - 113-21
AB  - PURPOSE: Chromosome abnormalities are an important prognostic factor in childhood 
      acute lymphoblastic leukemia (ALL). Recently, a subset of patients with 
      hyperdiploid ALL and trisomy of chromosomes 4 and 10 has been reported to have a 
      very favorable event-free survival. Rapid and accurate detection of these 
      patients will allow them to be treated with highly effective and relatively 
      nontoxic antimetabolite therapy. Because of inherent problems associated with 
      conventional cancer cytogenetics, we examined the efficacy of fluorescence in 
      situ hybridization (FISH) to identify this ALL subgroup. PATIENTS AND METHODS: 
      Fifty uncultured bone marrow specimens from children with newly diagnosed ALL 
      were examined for chromosomes 4 and 10 aneuploidy with FISH. These results were 
      compared with routine cytogenetics and DNA Index (DI). RESULTS: Interphase FISH 
      cytogenetics identified the abnormal cell line(s) in all cases in which 
      cytogenetics showed aneuploidy of chromosomes 4 and 10. In cases in which 
      cytogenetics was not informative, FISH identified the presence of an aneuploid 
      chromosome 4 and/or 10 cell line in concordance with the DI. CONCLUSIONS: FISH 
      interphase cytogenetics can accurately detect chromosome 4 and 10 aneuploidy in 
      leukemic cells. It is a rapid and clinically applicable technique that can 
      reliably identify childhood ALL cases who have trisomy of chromosomes 4 and 10 
      and who have very favorable event-free survival.
FAU - Martin, P L
AU  - Martin PL
AD  - Bowman Gray School of Medicine, Winston-Salem, North Carolina, USA.
FAU - Look, A T
AU  - Look AT
FAU - Schnell, S
AU  - Schnell S
FAU - Harris, M B
AU  - Harris MB
FAU - Pullen, J
AU  - Pullen J
FAU - Shuster, J J
AU  - Shuster JJ
FAU - Carroll, A J
AU  - Carroll AJ
FAU - Pettenati, M J
AU  - Pettenati MJ
FAU - Rao, P N
AU  - Rao PN
LA  - eng
GR  - CA-15989/CA/NCI NIH HHS/United States
GR  - CA-25408/CA/NCI NIH HHS/United States
GR  - CA-53128/CA/NCI NIH HHS/United States
GR  - etc.
PT  - Comparative Study
PT  - Journal Article
PT  - Multicenter Study
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Pediatr Hematol Oncol
JT  - Journal of pediatric hematology/oncology
JID - 9505928
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - *Aneuploidy
MH  - Child
MH  - *Chromosomes, Human, Pair 10
MH  - *Chromosomes, Human, Pair 4
MH  - DNA Probes
MH  - DNA, Neoplasm/*genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics
EDAT- 1996/05/01 00:00
MHDA- 1996/05/01 00:01
CRDT- 1996/05/01 00:00
PHST- 1996/05/01 00:00 [pubmed]
PHST- 1996/05/01 00:01 [medline]
PHST- 1996/05/01 00:00 [entrez]
AID - 10.1097/00043426-199605000-00004 [doi]
PST - ppublish
SO  - J Pediatr Hematol Oncol. 1996 May;18(2):113-21. doi: 
      10.1097/00043426-199605000-00004.