PMID- 8847957
OWN - NLM
STAT- MEDLINE
DCOM- 19961021
LR  - 20190701
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 57
IP  - 26
DP  - 1995 Nov 17
TI  - 5,6-Dihydroxyindole-2-carboxylic acid, a diffusible melanin precursor, is a 
      potent stimulator of lipopolysaccharide-induced production of nitric oxide by 
      J774 macrophages.
PG  - PL401-6
AB  - Pre-incubation of J774 murine macrophages with 5,6-dihydroxyindole-2-carboxylic 
      acid (DHICA), a diffusible intermediate in the biosynthesis of eumelanins, leads 
      to a marked increase in the levels of nitric oxide (NO) produced by 
      lipopolysaccharide (LPS)-induced NO-synthase (iNOS). The effect varies with DHICA 
      concentration being maximum at a concentration of 1 x 10(-6)M, and is suppressed 
      by the NOS inhibitor NG-monomethyl-L-arginine (L-NMMA). No stimulation is 
      observed when macrophages are exposed to DHICA after activation with LPS, 
      indicating that the indole does not affect the catalytic activity of iNOS. These 
      results point to a hitherto unrecognized role of DHICA as a chemical messenger 
      mediating interaction between active melanocytes and macrophages in epidermal 
      inflammatory and immune responses.
FAU - D'Acquisto, F
AU  - D'Acquisto F
AD  - Department of Experimental Pharmacology, University of Naples Federico II, Italy.
FAU - Carnuccio, R
AU  - Carnuccio R
FAU - d'Ischia, M
AU  - d'Ischia M
FAU - Misuraca, G
AU  - Misuraca G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Indoles)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Melanins)
RN  - 0 (Protein Precursors)
RN  - 27JT06E6GR (omega-N-Methylarginine)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 4790-08-3 (5,6-dihydroxy-2-indolylcarboxylic acid)
RN  - 94ZLA3W45F (Arginine)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
SB  - IM
MH  - Animals
MH  - Arginine/analogs & derivatives/pharmacology
MH  - Cell Line
MH  - Cells, Cultured
MH  - Diffusion
MH  - Enzyme Inhibitors/pharmacology
MH  - Indoles/*pharmacology
MH  - Lipopolysaccharides/*pharmacology
MH  - Macrophage Activation
MH  - Macrophages/drug effects/*metabolism
MH  - Melanins/*metabolism
MH  - Melanocytes/metabolism
MH  - Mice
MH  - Nitric Oxide/*biosynthesis
MH  - Nitric Oxide Synthase/antagonists & inhibitors
MH  - Protein Precursors/metabolism/pharmacology
MH  - *Salmonella typhi
MH  - omega-N-Methylarginine
EDAT- 1995/11/17 00:00
MHDA- 1995/11/17 00:01
CRDT- 1995/11/17 00:00
PHST- 1995/11/17 00:00 [pubmed]
PHST- 1995/11/17 00:01 [medline]
PHST- 1995/11/17 00:00 [entrez]
AID - 0024320595022442 [pii]
AID - 10.1016/0024-3205(95)02244-2 [doi]
PST - ppublish
SO  - Life Sci. 1995 Nov 17;57(26):PL401-6. doi: 10.1016/0024-3205(95)02244-2.