PMID- 8849672
OWN - NLM
STAT- MEDLINE
DCOM- 19970214
LR  - 20190905
IS  - 0340-2061 (Print)
IS  - 0340-2061 (Linking)
VI  - 194
IP  - 3
DP  - 1996 Sep
TI  - Epidermal growth factor receptor in adult human dorsal root ganglia.
PG  - 253-7
AB  - Transforming growth factor-alpha (TGFalpha) enhances neuronal survival and 
      neurite outgrowth in cultured dorsal root ganglia (DRG) sensory neurons. It binds 
      a membrane protein, denominated epidermal growth factor receptor (EGFr). EGFr has 
      been localized in developing and adult human DRG. However, it remains to be 
      elucidated whether all DRG neurons express EGFr or whether differences exist 
      among neuronal subtypes. This study was undertaken to investigate these topics in 
      adult human DRG using immunoblotting, and combined immunohistochemistry and image 
      analysis techniques. A mouse monoclonal antibody (clone F4) mapping within the 
      intracytoplasmic domain of EGFr was used. Immunoblotting revealed two main 
      proteins with estimated molecular masses of approximately/equal to 65 kDa and 170 
      kDa, and thus consistent with the full-length EGFr. Additional protein bands were 
      also encountered. Light immunohistochemistry revealed specific immunoreactivity 
      (IR) for EGFr-like proteins in most (86%) primary sensory neurons, the intensity 
      of immunostaining being stronger in the small- and intermediate-sized ones. 
      Furthermore, EGFr-like IR was also observed in the satellite glial cells of the 
      ganglia as well as in the intraganglionic and dorsal root Schwann cells. Taken 
      together, our findings demonstrate that EGFr, and other related proteins 
      containing the epitope labeled with the antibody F4, are responsible for the EGFr 
      IR reported in DRG. Furthermore, we demonstrated heterogeneity in the expression 
      of EGFr-like IR in adult human primary sensory neurons, which suggests different 
      responsiveness to their ligands.
FAU - Huerta, J J
AU  - Huerta JJ
AD  - Departamento de Morfologia y Biologia Celular, Universidad de Oviedo,Spain.
FAU - Diaz-Trelles, R
AU  - Diaz-Trelles R
FAU - Naves, F J
AU  - Naves FJ
FAU - Llamosas, M M
AU  - Llamosas MM
FAU - Del Valle, M E
AU  - Del Valle ME
FAU - Vega, J A
AU  - Vega JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Anat Embryol (Berl)
JT  - Anatomy and embryology
JID - 7505194
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Neurofilament Proteins)
RN  - 0 (S100 Proteins)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Adult
MH  - Antibodies, Monoclonal
MH  - Cell Size
MH  - ErbB Receptors/*analysis
MH  - Female
MH  - Ganglia, Spinal/*chemistry/cytology
MH  - Humans
MH  - Immunoblotting
MH  - Immunohistochemistry
MH  - Lumbosacral Region
MH  - Male
MH  - Neurofilament Proteins/analysis
MH  - Neuroglia/chemistry
MH  - S100 Proteins/analysis
EDAT- 1996/09/01 00:00
MHDA- 1996/09/01 00:01
CRDT- 1996/09/01 00:00
PHST- 1996/09/01 00:00 [pubmed]
PHST- 1996/09/01 00:01 [medline]
PHST- 1996/09/01 00:00 [entrez]
AID - 10.1007/BF00187136 [doi]
PST - ppublish
SO  - Anat Embryol (Berl). 1996 Sep;194(3):253-7. doi: 10.1007/BF00187136.