PMID- 8851195
OWN - NLM
STAT- MEDLINE
DCOM- 19961206
LR  - 20061115
IS  - 0047-1860 (Print)
IS  - 0047-1860 (Linking)
VI  - 44
IP  - 2
DP  - 1996 Feb
TI  - [The perspective on chromosomal painting (FISH)].
PG  - 133-7
AB  - With the recent advances in molecular biology, many sophisticated technologies 
      have been developed. The technique of fluorescence in situ hybridization (FISH) 
      is one of them, and is being widely used not only in research fields of molecular 
      biology but also for diagnosis in clinical laboratories. In this mini review, I 
      outline the representative techniques of FISH and its application to laboratory 
      medicine such as diagnosis of genitic and neoplastic diseases. Standard FISH 
      consists of two according to DNA probes used, namely whole chromosome painting 
      and centromere staining. Standard FISH is very useful for analysis of genetic 
      abnormalities. Whole chromosomal painting covering the whole chromosome 
      facilitates the rapid analysis of chromosome structural abnormalities, and 
      centromere staining using alphoid DNA probes allows rapid analysis of chromosome 
      numerical aberrations. Comparative genomic hybridization which has been recently 
      developed is a very attractive method to detect amplification and deletion of 
      chromosomes and genes in malignant tumors, and now is considered a powerful 
      strategy for search of new genes implicated in the development and progression of 
      neoplasm.
FAU - Sasaki, K
AU  - Sasaki K
AD  - Department of Pathology, Iwate Medical University, Morioka.
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PT  - Review
PL  - Japan
TA  - Rinsho Byori
JT  - Rinsho byori. The Japanese journal of clinical pathology
JID - 2984781R
SB  - IM
MH  - *Chromosomes, Human
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
RF  - 2
EDAT- 1996/02/01 00:00
MHDA- 1996/02/01 00:01
CRDT- 1996/02/01 00:00
PHST- 1996/02/01 00:00 [pubmed]
PHST- 1996/02/01 00:01 [medline]
PHST- 1996/02/01 00:00 [entrez]
PST - ppublish
SO  - Rinsho Byori. 1996 Feb;44(2):133-7.